Differential Expression of Angiogenic Factors in Skin of Patients with Psoriasis Vulgaris

Lakshna, S (2015) Differential Expression of Angiogenic Factors in Skin of Patients with Psoriasis Vulgaris. Masters thesis, Coimbatore Medical College, Coimbatore.

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Abstract

INTRODUCTION: Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation, abnormal differentiation, and inflammatory infiltration in epidermis and dermis. Angiogenesis or neovascularization refers to the formation of new capillary vessels from the existing vascular bed. Dermal microvascular expansion with abnormal orientation and dilatation of capillaries in the biopsies of the psoriatic skin revealed that the disease was angiogenesis dependent. AIM AND OBJECTIVES: 1. To analyse immunohistochemical expression of angiogenic factors Vascular endothelial growth factor, Von wille brand factor and CD 34 in skin biopsy of patients with psoriasis vulgaris and controls. 2. To compare the neovascularisation score of CD 34, VEGF and vWFr in skin biopsy of psoriasis vulgaris cases and controls. 3. To correlate the expression of angiogenic factors and psoriasis area and severity clinical index(PASI SCORE) which is a measure of clinical severity. MATERIALS AND METHODS: This is a case control study carried over a period of 15 months from April 2013 to July 2014.Thirty two psoriasis cases and thirty control healthy skin were studied. Biopsy specimen is taken from skin of newly diagnosed psoriasis and patients who have not taken any treatment two months prior to study. Histopathological examination of psoriasis vulgaris was confirmed. Immunohistochemical expression for VEGF (vascular endothelial growth factor), vonwillebrand factor and CD 34 was studied. RESULTS: VEGF expression in epidermis was significantly higher in icases when compared to control skin (p=<o.o1).CD 34 expression was significantly up regulated in cases when compared to controls.(p<0.01). Whereas only weak expression of vonwillebrand factor was observed in both cases and controls. Significant correlation between the expression of VEGF and PASI score (r=0.944;p<0.05), and expression of CD 34 and PASI score was observed (r=0.942;p<0.05). CONCLUSION: Significant overexpression of VEGF and CD 34 was noted in cases when compared to controls. The keratinocytes in the psoriatic skin lesions were recognized as a source of pro-angiogenic cytokines which induce angiogenesis, namely the vascular endothelial growth factor (VEGF) and other growth factors which promotes micrangiopathic modifications in psoriatic plaque. Angiogenesis plays an important role in genesis and development of psoriasis vulgaris. Therefore development of targeted anti- angiogenic therapy might be beneficial for this chronic disabling dermatological disease.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Differential Expression ; Angiogenic Factors ; Skin ; Psoriasis Vulgaris.
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:14
Last Modified: 23 May 2018 01:19
URI: http://repository-tnmgrmu.ac.in/id/eprint/7994

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