Comparison between Liquid Based Cytology and Conventional Cytopreparatory Methods in Body Cavity Fluids: A Study of 100 Cases

Swathy, P U (2015) Comparison between Liquid Based Cytology and Conventional Cytopreparatory Methods in Body Cavity Fluids: A Study of 100 Cases. Masters thesis, Stanley Medical College, Chennai.

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Abstract

AIM OF THE STUDY: To compare the morphology of cells by the two methods employed for processing of the fluids–“Conventional processing of fluids” and “Liquid based cytology technique” with regard to (a) cell yield, (b) cell morphology, (c) cell distribution and (d) background. METHODOLOGY: 100 samples of body cavity fluids comprising of 33 pleural fluids, 56 peritoneal fluids and 11 urine were analysed. Smears were prepared using Liquid based cytology and conventional methods. All smears were stained by hematoxylin and eosin. RESULTS: Liquid based cytology showed better cell yield as compared to conventional smears in pleural, peritoneal fluids and urine. (p<0.05)Cell morphology was better preserved by Liquid based cytology than conventional smears in pleural, peritoneal fluids and urine. (p<0.05) Liquid based cytology showed more uniform cell distribution as compared to conventional smears in pleural, peritoneal fluids and urine. These results showed a statistically significant difference between the two methods.(p<0.05)Liquid based cytology lyywas not comparable to conventional smears in terms of background because the results were not statistically significant. (p>0.05)This was true for pleural, peritoneal fluids and urine. CONCLUSION: Liquid based cytology was found superior to conventional smears in terms of cell yield, preservation of cell morphology and uniformity of cell distribution. Hence, Liquid based cytology can be preferred to conventional smears for cytologic examination of body cavity fluids. With regard to typing the characteristics of malignant effusions, more samples have to be analysed and a separate study is required.

Item Type: Thesis (Masters)
Uncontrolled Keywords: conventional method ; liquid based cytology ; pleural fluid ; peritoneal fluid ; urine
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:14
Last Modified: 22 May 2018 19:45
URI: http://repository-tnmgrmu.ac.in/id/eprint/7987

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