Immunohistochemical Analysis of Maspin Expression in Chronic Non Neoplastic and Neoplastic Colorectal Diseases: A Study of 70 Cases

Deepa, R (2015) Immunohistochemical Analysis of Maspin Expression in Chronic Non Neoplastic and Neoplastic Colorectal Diseases: A Study of 70 Cases. Masters thesis, Stanley Medical College, Chennai.

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Abstract

BACKGROUND: Maspin is found to be a member of serine protease inhibitor/ non inhibitor superfamily like plasminogen activator inhibitors 1 and 2 and alpha-1 antitrypsin. The gene for maspin is located on chromosome 18q21.3.It has been shown to be involved in processes that are important to both tumor growth and metastasis such as apoptosis, cell invasion and angiogenesis. This study is aimed to analyse maspin expression in chronic non neoplastic and neoplastic colorectal diseases. AIMS AND OBJECTIVES: 1. To study tumor progression in colorectal adenocarcinomas. 2. To evaluate maspin expression and its correlation with clinicopathologic parameters in chronic non neoplastic and neoplastic colorectal diseases. 3. To evaluate the prognostic value of immunohistochemical expression of maspin in chronic non neoplastic and neoplastic colorectal diseases. MATERIALS AND METHODS: A total of 70 cases including 10 controls (normal colonic mucosa) of non neoplastic and neoplastic colorectal diseases were used for immunohistochemical analysis of maspin expression. The cases included are Non specific colitis, Ulcerative colitis, colorectal adenomas and adenocarcinomas received in the Department of Stanley Medical College from the Department of Surgery in the year 2012-2013. The immunoreactivity to maspin was identified by staining of the cytoplasm. 100 cells were randomly selected and counted from 5 representative fields. RESULTS: In our study maspin expression was found to be increased in males,increasing depth of invasion, presence of lymphatic and vascular invasion and poor survival,thus showing increased expression with increase in aggressiveness of colorectal cancers. Also increased expression was found in carcinoma compared to normal colonic mucosa, non specific colitis and ulcerative colitis. No significance was found to be associated with increasing tumor size or differentiation of the tumor or presence of liver metastasis. Our study is comparable with other parallel studies where expression of maspin was studied in colorectal carcinoma. CONCLUSION: The expression of maspin with various clinicopathological parameters was analysed and its importance as a prognostic factor was assessed. To conclude in our study maspin was found to be associated with increasing aggressiveness of colorectal cancers.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Ulcerative colitis ; Adenoma ; Colorectal carcinoma ; immunohistochemistry ; Maspin.
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:14
Last Modified: 22 May 2018 19:35
URI: http://repository-tnmgrmu.ac.in/id/eprint/7985

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