Comparative Study of Hematoxylin & Eosin, Special Stains and Immunohistochemistry for Detection of Helicobacter Pylori in Both Neoplastic and Non-Neoplastic Gastric Lesions

Umasankari, L (2015) Comparative Study of Hematoxylin & Eosin, Special Stains and Immunohistochemistry for Detection of Helicobacter Pylori in Both Neoplastic and Non-Neoplastic Gastric Lesions. Masters thesis, Madras Medical College, Chennai.

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Abstract

AIMS AND OBJECTIVES: The role of Helicobacter Pylori in the pathogenesis of Gastritis-Peptic ulcer syndrome and its association with the development of upper gastrointestinal tract malignancy warrant efficient method for the identification of the bacteria in biopsy specimens. Four staining methods - Hematoxylin and Eosin, Giemsa, Toluidine Blue and Immunohistochemistry were compared to detect the presence of Helicobacter pylori. The prevalence of H.Pylori in gastric biopsies was also evaluated. METHODS: A total of 70 cases (40 cases of gastritis and 30 cases of gastric adenocarcinoma) were randomly selected for this study and all the four stains were applied. RESULTS: Helicobacter pylori infection showed an overall prevalence rate of about 67.1%. When compared with Immunohistochemistry, Sensitivity and specificity of Hematoxylin and Eosin was 57.45% and 100% respectively. Giemsa showed sensitivity of about 87.23% and specificity of about 100%. Toluidine blue showed sensitivity of about 78.72% and specificity of about 100%. CONCLUSION: Hence in the present study Giemsa was more reliable and cost effective stain when compared with Hematoxylin & Eosin, Toluidine blue and immunohistochemistry. However, Immunohistochemistry carries the highest level of sensitivity in the detection of Helicobacter Pylori especially when the density of organism is low and in clinically suspected cases of Helicobacter Pylori with negative Giemsa staining.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Helicobacter pylori ; Hematoxylin & Eosin ; Giemsa ; Toluidine blue ; Immunohistochemistry.
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:14
Last Modified: 22 May 2018 19:30
URI: http://repository-tnmgrmu.ac.in/id/eprint/7984

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