Conventional Cytological Smear Versus Liquid Based Preparation (E-PREP) in Non Gynaecological Samples

Maheswari, J (2015) Conventional Cytological Smear Versus Liquid Based Preparation (E-PREP) in Non Gynaecological Samples. Masters thesis, Madras Medical College, Chennai.

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Abstract

BACKGROUND AND OBJECTIVE: Fine needle cytology (FNC) has gained tremendous popularity in recent times among clinicians and pathologist. Liquid based cytology is a new technology for fine needle aspiration samples. It is used for mainly for cervical cancer screening, now also used for non gynaecolgical samples. E-PREP system is a Liquid based cytology processor with patent dual membrane filters. In this method able to collect large number of cells and make a monolayer preparation of cells with good cytological details. In LBP easier collection and transport of samples, standardized preparation, adequate cellularity, rapid fixation, even and monolayer distribution of cells, good preservation of cell morphology and increase clarity of nuclear feature, decreased obscuring background elements, decreased air drying artefacts. Disadvantages of LBP are decreased and altered background material like necrosis, blood and inflammation, decreased and altered extracellular elements like mucin, colloid and stroma, disrupted cellular architecture like fragmentation of papillae, size of the cell smaller than conventional preparation. In this study thyroid, breast and lymphnode lesions (each 30 cases) are compared with both techniques of FNAC and Liquid based preparation (E-PREP) in non gynaecological samples. MATERIALS AND METHODS: This study is a prospective study conducted at Goschen Institute of Pathology, Madras Medical College and Rajiv Gandhi Government General Hospital Chennai, during the year 2014 (February 2014 to July 2014). This study included samples obtained from 90 patients who attended for cytology department for FNAC of thyroid, breast and lymphnode lesions. Collection of clinical data of patients attending the cytology department for FNAC for thyroid, breast and lymphnode lesions and preparing CSs & LBP. RESULTS: Smears were prepared by both methods. On analysing smears in thyroid lesion adequate smears more in CS method, superior quality smears more from LBP method and equal number diagnostically unsuitable smears from each methods of smear preparation, in breast lesions adequate smears more in LBP method, superior quality smears more from CS method and diagnostically unsuitable smears more from LBP method of smear preparation and in lymphnode lesion adequate smears more in LBP method, superior quality smears more from CS method and diagnostically unsuitable smears more from LBP method of smear preparation. On analysing and comparing average score obtained by both methods (CS & LBP) in thyroid, breast and lymphnode lesions and the P value calculated by Pearson Chi-Square test, the difference was found to be statistically insignificant P> 0.05. CONCLUSION: The decision to make, use either Conventional method or LBP may be depends on basis of nature of the lesion ( solid or cystic) and other ancillary tests to perform in the sample &each method has its own advantages and disadvantages and both methods can be combined to obtain a superior quality smears and lower the failure rates.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Fine needle aspiration cytology (FNAC) ; Conventional smear (CS) ; Liquid based preparation (LBP) ; thyroid, breast ; lymphnode.
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:13
Last Modified: 22 May 2018 19:15
URI: http://repository-tnmgrmu.ac.in/id/eprint/7980

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