Anuradha, B (2011) Evaluation of Antimicrobial Effect of Octenidine, Triclosan and Chlorhexidine against E.faecalis in Root Canal Disinfection: An Exvivo Study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
The present study evaluated the antimicrobial efficacy of octenidine, triclosan and chlorhexidine against Enterococcus faecalis in root canal disinfection. 50 single rooted pre-molar teeth wih no caries and root fillings were collected. The dentine blocks were standardized by decoronating the teeth to measure 7mm long segments. The root canals were enlarged to size 40 using K files. The root surface were coated with nail varnish and was allowed to dry at the room temperature for 1hr and subsequently the root apex was sealed using light cure composite resin. Following sterilization of the blocks, they were infected with pure cultures of E.faecalis, which was grown in Muller-Hinton agar for a period of 7 days. The broth was changed every alternate day to maintain viable cultures. The specimens were divided into 5 distinctive groups containing 10 teeth each: Group I with Octenidine, (0.1%), Group II with Chlorhexidine (2%), Group III with Triclosan,(99.0%), Group IV with NaOCL (5%) (control), Group IV with no irrigation (negative control). Each block was carefully removed from the broth and held with artery forceps. The block was irrigated with 3ml of the irrigant for 1 min. After irrigation, the dentine debris were harvested from the dentine blocks using Gates Glidden drills No.2 and No.3. The antibacterial assessment was carried out by finding out the MIC and MBC values by serial dilution method, the colony forming unit on Muller-Hinton agar plate and the proportions of viable bacteria under epifluorescence microscope using Syto9 dye. Statistical analysis comparing the inter-group was done using MANN-WHITNEY U test and KRUSKAL-WALLIS test. From the results it was observed that the antimicrobial efficacy of 0.1% Octenidine was the most, followed by 2% Chlorhexidine, 99.0% Triclosan and 5% Sodium hypochlorite against E.faecalis. Thus the irrigants do play a significant role in creating a “bacteria-free” root canal system. CONCLUSION: Within the parametric limitations of this in vitro study, it can be inferred that: 1. The MIC values of Octenidine hydrochloride, Chlorhexidine digluconate and triclosan, were identical as compared to that of MBC, thereby revealing a good bactericidal property. Octenidine which showed good antibacterial property, in terms of very minimal concentration being required. 2. Results of the in-vitro evaluation by determination of Minimum Inhibitory concentration (MIC) and Minimal Bactericidal concentration (MBC) values correlated very well as compared with the mono infection using Enterococcus faecalis in an ex-vivo human dentine block model. 3. The mono infection using Enterococcus faecalis in the dentine block model worked very well with a short incubation period of 7 days, and showed efficacy in view of a statistically significant reduction in the colony count after irrigation for 3 mins. 4. The special staining dye Syto9 showed significant reduction in the proportion of viable bacteria thereby proving the efficacy of the 3 dental irrigants. This method however, is technically very sensitive and not within reach of all laboratories. 5. Octenidine which is not yet evaluated widely as an irrigant showed the maximum efficacy, against E.faecalis thus proving to be a potent irrigant. Further in vivo studies are required to evaluate the tissue dissolving properties and also their safety in dental practice.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Antimicrobial Effect of Octenidine ; Triclosan ; Chlorhexidine ; E.faecalis ; Root Canal Disinfection ; Exvivo study. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Kambaraman B |
| Date Deposited: | 14 May 2018 03:26 |
| Last Modified: | 14 May 2018 03:26 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/7810 |
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