Rakesh, R Rajan (2011) Effect of Irrigation of Sodium Hypochlorite at Different Temperatures and Concentration on the Elimination of Enterococcus Faecalis from Rootcanals: An In Vitro Study. Masters thesis, J.K.K. Nataraja Dental College, Komarapalayam.
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Abstract
Sixty single rooted anterior teeth were collected, cleaned and stored in normal saline. They were standardized to a length of 15mm and the root canals preparation was done using Protaper instruments as per the manufacturer’s instructions. The teeth were then divided into groups and inoculated with Enterococcus faecalis. Followed by irrigation with varying concentrations of Sodium hypochlorite (1%, 3% and 5%) at temperatures of (240Celsius, 370Celsius,450Celsius). Sampling was done by inserting paper points followed by culturing. After 48hrs of incubation typical Enterococcus faecalis colonies were counted in all plates, results tabulated and statistically analyzed. CONCLUSION: On completion of the study on the effect of varying concentration of sodium hypochlorite at three different temperatures on Enterococcus faecalis within the canals. The following conclusions were made: 1. 5% sodium hypochlorite at 450Celsius was found to be the most effective irrigant used. 2. Statistical analysis with ANOVA at 5% level of significance showed there is a significant difference between 5% sodium hypochlorite at 450Celsius and other two groups of 5% sodium hypochlorite at 370Celsius and 240Celsius which means that 5%sodium hypochlorite at 450 is better. 3. Based on the results of the study chair side pre-warming sodium hypochlorite before use with a temperature controlled device is recommended. 4. 5% Sodium hypochlorite at 240Celsius and 370Celsius were also able to inhibit the growth of Enterococcus faecalis efficiently in comparison to the control group. Effectiveness of sodium hypochlorite as an irrigant at lower concentration could be enhanced by increasing the temperature. 5. The effect of varying concentrations of sodium hypochlorite as an irrigant at varying temperature in a clinical setting should be evaluated.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Irrigation ; Sodium Hypochlorite ; Different Temperatures ; Concentration ; Elimination ; Enterococcus Faecalis ; Rootcanals ; In Vitro study. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Kambaraman B |
| Date Deposited: | 14 May 2018 01:22 |
| Last Modified: | 14 May 2018 01:22 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/7805 |
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