Jothi Latha, S (2010) Comparative Evaluation of Antimicrobial Activity of Triphala, Terminalia Chebula & Chlorhexidine on Enterococcus Faecalis: An Invitro Study. Masters thesis, Tamilnadu Government Dental College and Hospital, Chennai.
|
Text
240402010jothi_latha.pdf Download (4MB) | Preview |
Abstract
Endodontic treatment is targeted towards reduction and elimination of microorganisms from the root canal system. Intracanal medicaments can aid in achieving this objective. This study evaluates the antimicrobial activity of Triphala & T. chebula against E. faecalis, in comparison with 2% CHX. Minimal inhibitory concentration of the alcoholic extracts of Triphala & T. chebula was found using agar-dilution method. Agar diffusion method was used to compare the zone of inhibition produced by the herbal extracts & CHX. All the three showed significant antimicrobial activity when compared with the control groups. The herbal extracts showed significant difference when compared with CHX. Activity on biofilms formed in teeth samples were studied using a modification of the model proposed by Haapasalo & Orstavik. The dentin blocks were infected with E. faecalis for 21 days, then they were divided into 4 groups based on the medication given, as Group I – Saline (Infected dentine blocks), Group II – Triphala, Group III – T. chebula & Group IV – CHX. The medicaments were maintained for 7 days. Uninfected dentine samples served as negative control in Group V. At the end of 1 week, antimicrobial efficiency was assessed at the depths of 200μm & 400μm, by colony count (CFU / ml) & optical density (OD) values. All the 3 medicaments showed statistically significant antimicrobial activity. Assessment of the antimicrobial activity using OD values did not show any statistically significant difference among the test groups at 200μm. But there was statistically significant difference between the herbal extracts and CHX at 400μm. On microbial culturing, Triphala & T. chebula showed 100% inhibition of growth of bacteria in all the samples. CHX showed 100% inhibition in all the samples at 200μm and in 50% of the samples at 400μm. Assessment of the colony count (CFU / ml) showed significant antimicrobial activity for all of the medicaments. The difference shown between the extracts & CHX in the colony count was not statistically significant. But complete inhibition shown by the herbal extracts in all the samples may have some clinical significance. The results showed the potential of Triphala and T. chebula to be used as intracanal medicament. CONCLUSION: Results of this study indicate that E. faecalis biofilm present in the root canal system can be eliminated or reduced with intracanal medicaments. The herbal extracts of Triphala, T. chebula have the potential to be used as intracanal medicaments, as the already established 2% of CHX. They also have antimicrobial action on the E. faecalis biofilm penetrated within the dentinal tubules. The herbal extracts may have superior antimicrobial activity even at deeper dentine depths. Thus, this study opens a new avenue of trying herbal extracts as intracanal medicaments to eliminate the microorganism in the root canal system and aid in long term success in endodontics.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Comparative Evaluation ; Antimicrobial Activity ; Triphala, Terminalia Chebula ; Chlorhexidine ; Enterococcus Faecalis ; Invitro study. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Kambaraman B |
| Date Deposited: | 14 May 2018 00:28 |
| Last Modified: | 14 May 2018 00:28 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/7797 |
Actions (login required)
 |
View Item |
