Rajesh, N (2012) Evaluation of mRNA Expression of the Transcription Factors of Th1 and Th2 subsets (T- bet and GATA-3) in Periodontal Health and Disease. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: T cells play a prominent role in the etiopathogenesis of periodontal disease. The Th1/Th2 paradigm which has been used to explain the pathogenic mechanisms involved in periodontal disease, was based on the cytokine profile. Establishment of Th1 and Th2 subsets largely depends on its transcriptional regulation. AIMS & OBJECTIVES: The aim of this study is to evaluate the mRNA expression of the transcription factors of Th1 and Th2 subsets namely T-bet and GATA-3 respectively, from the gingival tissue samples of periodontally healthy and diseased patients. MATERIALS AND METHODS: Ten gingival tissue biopsy samples from each group of patients (periodontal health-Group A and periodontal disease- Group B) were obtained. The samples were processed for real time reverse transcription polymerase chain reaction to detect the mRNA expression of T-bet and GATA-3 in periodontal health and disease samples. RESULTS: The mRNA expression of the master transcription factor of Th1 subset, T- bet showed a marginal increase of about 1.31 fold, which was insignificant, while the GATA-3 levels showed a 4.39 fold decrease from disease to health. CONCLUSION: The results of this study indicates that the advanced periodontal lesions are more likely to be dominated by Th1, rather than Th2.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | T box expressed activated T cell (T-bet) ; Trans-acting T-cell-specific transcription factor GATA-3 (GATA-3) ; real time RT - PCR |
| Subjects: | DENTAL > Periodontology |
| Depositing User: | Kambaraman B |
| Date Deposited: | 13 May 2018 09:48 |
| Last Modified: | 13 May 2018 09:48 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/7768 |
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