Evaluation of Circulatory and Salivary Levels of Heat Shock Protein 60 in Periodontal Health and Disease

Ramya Nethravathy, R (2011) Evaluation of Circulatory and Salivary Levels of Heat Shock Protein 60 in Periodontal Health and Disease. Masters thesis, Ragas Dental College and Hospital, Chennai.


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BACKGROUND: Self antigens such as Heat shock protein 60 (HSP 60) have recently been implicated in the periodontal disease pathogenesis. There is scant evidence regarding HSP 60 in circulation and saliva following periodontal disease and its possible relation to systemic inflammation. MATERIALS AND METHODS: Forty five peripheral blood samples and saliva samples were collected from two groups of patients (periodontal health-Group A and periodontal disease- Group B). The serum, cell lysates, and saliva samples were used to detect and compare the HSP 60 levels in both the groups by Enzyme Linked Immuno Sorbent Assay (ELISA) technique. Statistical analysis was done using the student t test and Pearson’s correlation. RESULTS: Circulatory HSP 60 significantly increases in periodontal disease compared to health. There was a significant correlation between the total circulating HSP 60 but not cell lysate and serum levels in periodontal disease. There was no significant association between salivary HSP 60 and periodontal disease or systemic inflammation. CONCLUSION: Circulating HSP 60 levels seem to play role in the systemic inflammatory state produced by periodontal disease. Salivary HSP 60 may not be used as a surrogate to determine systemic inflammation.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Heat Shock Protein (HSP 60) ; Periodontitis ; Peripheral blood ; Cell lysates ; Enzyme Linked Immuno Sorbent Assay (ELISA).
Subjects: DENTAL > Periodontology
Depositing User: Kambaraman B
Date Deposited: 13 May 2018 09:19
Last Modified: 13 May 2018 09:19
URI: http://repository-tnmgrmu.ac.in/id/eprint/7766

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