Evaluation of Circulatory and Salivary Levels of IL-17 in Periodontal Health and Disease

Najumudeen, A (2011) Evaluation of Circulatory and Salivary Levels of IL-17 in Periodontal Health and Disease. Masters thesis, Ragas Dental College and Hospital, Chennai.

[img]
Preview
Text
240202311najumudeen.pdf

Download (1MB) | Preview

Abstract

BACKGROUND: This study was performed to evaluate the circulatory and salivary levels of Interleukin-17 (IL-17) in periodontal health and disease and to correlate it to serum high sensitivity- C reactive protein (hs-CRP) levels. MATERIALS & METHODS: Peripheral blood and saliva samples were collected from forty periodontal healthy and diseased patients who were recruited for the study in Ragas Dental College and Hospital. Serum was separated from patient’s blood and whole unstimulated saliva was centrifuged and subjected to sandwich ELISA analysis for quantification of serum and salivary IL-17 levels. Serum hs-CRP level was detected by the immunoturbidimetric method. Statistical analysis to evaluate the serum and salivary levels of IL-17 was done using the independent sample student ‘t’ test and Pearson’s correlation analysis to correlate the serum and salivary IL-17 levels to the hs-CRP. RESULTS: The serum IL-17 level in the periodontally diseased group was decreased when compared to the healthy group (D = 23.77 ± 0.43; H = 25.66 ±1.06), which was not statistically significant (P = 0.67; P > 0.05). However, there was a statistically significant (P = 0.038; P < 0.05) decrease in the salivary IL-17 levels in periodontally diseased group when compared to the healthy group (D = 22.24 ± 0.29; H = 24.63 ± 0.93). There was a statistically significant increase (P = 0.02; P < 0.05) in hs-CRP levels in periodontal disease when compared to health (D = 2.25 ± 0.29; H = 1.16 ± 0.03). There was a statistically significant correlation (P = 0.02; P < 0.05) between the serum and salivary levels of IL-17 in periodontal disease (serum = 22.24 ±0.29; saliva = 23.77 ± 0.43), but there was no statistically significant correlation between the serum and salivary levels of IL-17 to that of hs-CRP, in the periodontal disease group (IL-17 and circulating hs-CRP levels, P =0.706 and/or salivary IL-17 and circulating hs -CRP levels, P = 0.54). CONCLUSION: Salivary IL-17 was significantly decreased in periodontal disease when compared to serum IL-17 levels did not correlate with hs-CRP levels.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Helper T Cell 17 (Th17) ; Interleukin 17 (IL-17) ; Periodontitis ; periodontal pathogenesis ; Enzyme Linked Immuno Sorbent Assay (ELISA).
Subjects: DENTAL > Periodontology
Depositing User: Kambaraman B
Date Deposited: 13 May 2018 08:40
Last Modified: 13 May 2018 08:40
URI: http://repository-tnmgrmu.ac.in/id/eprint/7763

Actions (login required)

View Item View Item