The Association of Single Nucleotide Polymorphism (SNP) in Promoter Region -607 C-A of IL-18 Coding Region with Chronic Periodontitis

Sivsankar, P (2011) The Association of Single Nucleotide Polymorphism (SNP) in Promoter Region -607 C-A of IL-18 Coding Region with Chronic Periodontitis. Masters thesis, Tamilnadu Government Dental College and Hospital, Chennai.

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Abstract

BACKGROUND: Periodontal disease is a chronic inflammatory disease resulting in destruction of tooth supporting apparatus. Although the disease is microbial in origin destruction of hard and soft tissues of periodontium occurs as an exaggerated host response. 50% variation in inflammatory response/cytokine production is thought to be related to genetic factors such that genetic risk markers are now recognized as important risk determinants of periodontal disease. IL -18 is a pleiotrophic cytokine with multiple biological activities involved in regulation of both the innate and the aquired immune responses through various biological pathways. The interleukin -18 molecule shares its structural homogeneity with interleukin 1 receptor family, TNF-α. Interleukin -18 plays a role in periodontal disease susceptibility, initiation and progression through the Th1/Th2 cytokine profile. AIM OF THE STUDY: The aim of the study is to the distribution of single nucleotide polymorphism, in promoter region -607 C-A of IL-18 coding region, in the study population and to correlate the association of single nucleotide polymorphism (SNP) in promoter region -607 C-A of IL-18 coding region with chronic periodontitis. MATERIALS AND METHODS: Genomic DNA was obtained from peripheral blood samples in 100 periodontally healthy subjects (control group), 100 subjects with generalized chronic periodontitis (study group) and promoter region -607 C-A of IL-18 coding region was amplified by polymerase chain reaction, and the polymorphism was analyzed by restriction endonuclease cleavage. The alleles were detected by agarose gel electrophoresis and visualised with ethidium bromide. RESULTS: The percentage distribution of allele frequency for A and C allele among control and study group is statistically insignificant as the p values is greater than 0.05 (p value 0.932). Also the distribution of genotypes for the IL-18 gene polymorphism at position-607 among the control study group is insignificant whose p value is 0.854. CONCLUSION: Susceptibility for periodontitis is not influenced by the SNP at -607of IL 18 gene that have been investigated in this study and we find no association between them.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Single Nucleotide Polymorphism (SNP) ; Promoter Region -607 C-A ; IL-18 Coding Region ; Chronic Periodontitis.
Subjects: DENTAL > Periodontology
Depositing User: Kambaraman B
Date Deposited: 13 May 2018 04:09
Last Modified: 13 May 2018 04:09
URI: http://repository-tnmgrmu.ac.in/id/eprint/7756

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