Indhu, K (2015) Oxidative and Inflammatory Status in Type-2 Diabetes Mellitus Patients With and Without Cardiac Complications. Masters thesis, PSG Institute of Medical Sciences and Research, Coimbatore.
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Abstract
BACKGROUND AND OBJECTIVE: Diabetes mellitus, a heterogeneous collection of metabolic disorders may be due to reduced insulin secretion or decrease in the effectiveness of secreted insulin or a combination of both. Increased oxidative stress and inflammation contributes to the development and progression of diabetes and its complications. Diabetes aggravates other co-morbidities such as obesity, hypertension and dyslipidemia which also increase the risk for Cardio Vascular Disease. High-sensitivity Creactive protein (hs-CRP) an acute phase protein is considered to be predictor of future coronary events. Thiols constitute the major portion of the total body antioxidants and they play a significant role in defense against reactive oxygen species. Objective of this study was to estimate the levels of hsCRP and protein thiols in type 2 diabetic patients with and without cardiac complications .In addition this study also tries to establish a correlation between glycated haemoglobin, hsCRP and protein thiols in both the study groups. MATERIAL AND METHODS: This is a cross sectional study. Type-2 diabetic patients belonging to the age group of 30-75 years were selected from Diabetology OPD and Cardiology ward. HbA1c and plasma hsCRP were estimated in 60 type-2 diabetic patients without complications and 60 type-2 diabetic patients with cardiac complications. Type-2 diabetic patients with other complications were excluded. Plasma total protein thiols was estimated spectrophotometrically by using Dinitrobenzene (DTNB)-Ellman’s method, plasma hsCRP was measured by particle enhanced turbidimetric assay and HbA1c by turbidimetric inhibition immunoassay. RESULTS: There was no statistically significant difference between the two study groups with regard to age and gender. The mean hsCRP activity in type-2 diabetic patientswithout complications was 0.2885 ±0.26758 and with cardiac complications was 3.1970±5.83335 which was statistically significant with the p value of 0.000187. The p value of HbA1c levels between the two groups was found to be 0.047 which was also statistically significant. There was no statistical significance (p value, 0.530) between the two groups with regard to protein thiols. Further, the Pearson correlation analysis showed a significant positive correlation between the blood levels of HbA1c with the plasma total protein thiols (p value < 0.001) and hsCRP (p value 0.018). In addition there is a very significant positive correlation between the plasma levels of hsCRP and the plasma total protein thiols with a p value of < 0.001. INTERPRETATION AND CONCLUSION: Increased HbA1c and plasma hsCRP in type 2 diabetic patients with cardiac complications can be attributed to the fact that oxidative stress and inflammation due to persistent hyperglycemia play a major role in the pathogenesis of diabetic complications. The increase in thiol levels along with hsCRP is due to the increased synthesis to compensate for the loss incurred during neutralization of the oxidants in Type 2 diabetic patients.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Diabetes mellitus ; Cardiovascular disease ; Atherosclerosis ; Oxidative Stress ; Inflammation ; High sensitivity C reactive protein ; Protein thiols. |
| Subjects: | MEDICAL > Biochemistry |
| Depositing User: | Punitha K |
| Date Deposited: | 06 May 2018 16:17 |
| Last Modified: | 06 May 2018 16:17 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/7559 |
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