Morphology and Distribution of Dendritic Cells in Normal and Carcinoma Cervix

Mohana Priya, E (2015) Morphology and Distribution of Dendritic Cells in Normal and Carcinoma Cervix. Masters thesis, Christian Medical College, Vellore.

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Abstract

OBJECTIVES: To study and compare the morphology, size and distribution of CD1a positive Langerhans Cells (LCs) in the ectocervical epithelium of the normal cervix, carcinoma of cervix and normal cervix associated with other genital carcinomas. METHODS: Normal and cancerous ectocervical tissues, obtained after informed consent, from 47 women undergoing hysterectomies, were processed for immunohistochemical staining with CD1a marker and studied by light microscopy. The morphology and distribution of the CD1a positive LCs were studied. The difference in the total number of LCs and different types of CD1a positive LCs per mm length of ectocervical epithelium were compared between the various groups using the Kruskal Wallis test and between pairs of groups using the Mann Whitney U test. The mean diameters of LCs were compared between the groups using ANOVA and pairs of groups using the Scheffe multiple comparison test. RESULTS: There were significantly fewer CD1a positive LCs in squamous cell and adenocarcinoma of cervix than in normal. An additional previously unclassified variety of LCs having no processes was noted and this was the predominant type in carcinoma cervix. Cells having single processes were significantly higher in normal cervix as compared to cervical carcinomas. The diameters of CD1a positive LCs were significantly higher in normal cervix than in carcinoma cervix. This data may shed new insights into the role of LCs in the disease process of cervical carcinomas. CONCLUSIONS: 1. All the cervical specimens showed the presence of CD1a positive LCs. 2. Regional variation was noted in the distribution of LCs. 3. In the normal ectocervix, LCs were predominantly present in the suprabasal and intermediate layers of stratified squamous epithelium. 4. In CIN I LCs were predominantly present in the suprabasal layer of the ectocervical epithelium. 5. In case of carcinoma of cervix, LCs were seen either in the entire thickness, in the suprabasal or mid-epithelial layers, or infrequently, in the superficial layers of the ectocervical epithelium. 6. In addition to the five types of LCs classified by Figueroa and Caorsi (26), LCs with no processes were also noted in all the specimens. 7. While in the normal cervix, CIN I and normal cervix associated with other genital carcinoma LCs predominantly had a single process (Type I), cells with no processes were predominant in the squamous cell carcinoma and adenocarcinoma groups. 8. LC migration through the basement membrane was noted in all the five categorized groups. 9. A large number of LCs was noted associated with lymphocytic infiltrations in both epithelium and lamina propria. 10. LCs were found in close association with the capillaries and HEVs in the lamina propria of cases with squamous cell carcinoma of the cervix. 11. The mean number of CD1a positive LCs per mm length of ectocervical epithelium was highest in CIN I and in normal cervix when compared with carcinoma of cervix and normal cervix associated with other genital carcinomas. 12. The number of CD1a positive LCs was significantly lower in squamous cell and adenocarcinoma of cervix as compared to normal cervix. 13. The number of type I CD1a positive LCs was significantly higher in normal cervix as compared to squamous cell carcinoma of cervix. 14. The number of type I CD1a positive LCs was significantly higher in normal cervix as compared to adenocarcinoma of cervix. 15. Although the number of LCs was sparse in the intact epithelium away from tumour areas, dense infiltrations of LCs were noted in the tumour areas in poorly differentiated squamous cell carcinoma of cervix. 16. The diameters of CD1a positive LCs were significantly higher in normal cervix as compared to squamous cell and adenocarcinoma of cervix and normal cervix associated with other genital carcinomas. 17. The diameters of CD1a positive LCs were significantly greater in CIN I as compared to adenocarcinoma of cervix and normal cervix associated with other genital carcinomas. 18. The diameters of CD1a positive LCs were significantly higher in squamous cell carcinoma of cervix as compared to adenocarcinoma of cervix and normal cervix associated with other genital carcinomas.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Langerhans cells ; CD1a ; morphometry and squamous cell carcinoma.
Subjects: MEDICAL > Anatomy
Depositing User: Punitha K
Date Deposited: 05 May 2018 03:33
Last Modified: 05 May 2018 03:33
URI: http://repository-tnmgrmu.ac.in/id/eprint/7471

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