Transmission Electron Microscopic Study of the Microvasculature of the Human Postpartum Fallopian Tube

Minu Rekha, B (2015) Transmission Electron Microscopic Study of the Microvasculature of the Human Postpartum Fallopian Tube. Masters thesis, Christian Medical College, Vellore.

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Abstract

OBJECTIVES: To quantify the mucosal microvessels and intraepithelial lymphocytes (IELs) in the human postpartum Fallopian tube under light microscope and to look for the presence of high endothelial venules and their association with antigen presenting cells under transmission electron microscope. METHODS: The human postpartum Fallopian tube samples were processed for electron microscopy. Semithin sections, stained with 1% toluidine blue and ultrathin sections, stained with uranyl acetate and Reynold’s lead citrate were obtained. Semithin sections were examined under Olympus BX43 light microscope connected to a computer with cellSens image analysing software. Using which, the number of mucosal microvessels and intraepithelial cells were quantified. Ultrathin sections were examined under transmission electron microscope (TECHNAI T12 SPIRIT) wherein, the microvessels were studied and mucosal mast cells quantified. Descriptive analysis, spearman’s rho correlation and simple regression analysis of the data were obtained using SPSS version 17.0. RESULTS: The mean number of mucosal arterioles, capillaries, high endothelilal venules (HEVs) and venules per mm2 were 29.23 + 6.87, 161.38 + 7.05, 19.23 + 7.05 and 39.50 + 9.96 respectively. The mean number of intraepithelial lymphocytes per 100 mm length of epithelium was 771.30 + 300.85. There was a significant positive correlation between the number of IELs and HEVs (p<0.001) and IELs regressed on HEVs with the regression coefficient of 42.002. The mean number of mucosal mast cells per 1.5625 mm2 was 40.30 + 15.68. Migration of lymphocytes through mucosal HEVs and the association of antigen presenting cells (dendritic cells and macrophages) with microvessels were also observed. The presence of IELs, HEVs and the manner of interaction of IELs with the neighbouring epithelial cells proves that Fallopian tube is a member of MALT. It is hoped that these data from the study may provide some insights into the understanding of microvessels in the Fallopian tube under the influence of hormones during pregnancy and their association with immune cells.

Item Type: Thesis (Masters)
Uncontrolled Keywords: High endothelial venules ; intraepithelial lymphocytes ; mast cells ; telocytes ; clear cell variant.
Subjects: MEDICAL > Anatomy
Depositing User: Punitha K
Date Deposited: 05 May 2018 03:27
Last Modified: 05 May 2018 03:27
URI: http://repository-tnmgrmu.ac.in/id/eprint/7470

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