Gayatri, Balachandran (2013) Curcumin potentiates the chemotherapeutic efficacy of Cisplatin in 7,12 dimethylbenzanthracene induced oral cancer in Syrian golden hamsters. Masters thesis, Christian Medical College, Vellore.
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Abstract
OBJECTIVES: The objective of this study was to induce oral squamous cell carcinoma in Syrian golden hamsters with 7, 12 dimethylbenzanthracene and to measure the reduction in tumour area and gross tumour volume and proliferation of the tumour following oral administration of Curcumin, and measure its potentiating action on that of parenterally administered Cisplatin. METHODS: A comparative animal study was designed using Syrian golden hamsters. There were four study arms with 6 animals each, adding upto 24 animals. 7, 12 dimethylbenzanthracene was employed as a topical carcinogen, applied to the buccal pouch of all 24 hamsters thrice a week for 12 weeks. Following tumour induction, the subjects were divided into four groups as follows: A – Control group, B – Curcumin alone group, C – Cisplatin alone group, D – Curcumin along with Cisplatin group. Curcumin was administered enterally at a dosage of 80mg/kg/day for six weeks, while Cisplatin was delivered by intraperitoneal injection once a week for four weeks, each dose amounting to 40 mg/m2 (body surface area). Following this, the animals were sacrificed and the buccal pouch submitted for microscopic analysis. The outcomes studied were absolute and percentage reduction in tumour area (from pre- to post-intervention), tumour volume post-intervention and proliferative index of the tumour based on mib-1 staining on histology. RESULTS: There was 100% tumour incidence in the control arm. The maximum reduction in area of tumour was in Group D (77.22%), as compared to 47.82% in Group C and 16.08% in Group B. The interaction was, thus, synergistic. This difference was statistically significant (p value <<<). The lowest tumour volume and lowest proliferative index on histology was also observed in Group D, proving that maximum tumour inhibition was achieved with combination therapy as opposed to either agent alone.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Curcumin potentiates ; chemotherapeutic efficacy ; Cisplatin ; 7,12 dimethylbenzanthracene ; oral cancer ; Syrian golden hamsters. |
| Subjects: | MEDICAL > General Surgery |
| Depositing User: | Devi S |
| Date Deposited: | 04 May 2018 04:13 |
| Last Modified: | 04 May 2018 06:08 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/7435 |
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