Shalin, K (2017) Pharmacognostical and Phytochemical Studies on Leaves of Erythrina Variegata Linn., and Evaluation of its Effect on Metabolic Symptoms of Experimentally Induced Polycystic Ovarian Disease. Masters thesis, College of Pharmacy Madras Medical College, Chennai.
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Abstract
INTRODUCTION: The field of Pharmacognosy has well advanced in the past centuries. Natural products from the medicinal plant source are the main cast of modern drugs in clinical use. AIM AND OBJECTIVES: 1. To objective of the study is to explore the Phamacognostical, Phytochemical and Pharmacological profile on the leaves of Erythrina variegata Linn., 2. Collection and Authentication of the plant specimen. 3. Establishing Pharmacognostical profile of the plant. 4. Extraction of plant material by using, Soxhlet apparatus with solvents of increasing polarity such as Petroleum ether > Chloroform > Ethyl acetate > Ethanol and Aqueous (cold maceration). 5. Phytochemical screening and determination of bioactive constituents. 6. Evaluation of Polycystic Ovarian Disease activity by In – vitro and In – vivo models. 7. Isolation of compound using Column Chromatography. 8. Survey of Polycystic Ovarian Disease. SUMMARY AND CONCLUSION: Herbal medicines are found to be effective in treatment of various ailments but the major lacuna is lack of proper scientific validation. Hence the present study is aimed at investigating the selected plant Erythrina variegata Linn., for the Polycystic Ovarian Disease. The plant Erythrina variegata Linn., belonging the family Fabaceae, is claimed to be useful for Emmenagogue, but the claim has not been scientifically validated. Authentication of the plant material plays a key role in Pharmacognostical studies. The plant Erythrina variegata Linn., was collected from Karur district, Pillapalayam village and Authenticated by Prof. P. Jayaraman, Ph.D., Plant Anatomy Research Centre, Chennai. The parameters studied were Macroscopy, Microscopy, Powder microscopy, Leaf constants, Linear measurements, Histochemical studies and Physiochemical constants to establish data for proper authentication and detection of adulterants. The qualitative and quantitative analysis were carried out to identify inorganic elements present in the plant. The qualitative and quantitative analysis of toxic heavy metals like Cadmium, Arsenic, Lead and Mercury were within the WHO limits and ensure the safety of the drug. In Phytochemical analysis, extraction is the first step involved. The coarse powder was extracted by Petroleum ether, Chloroform, Ethyl acetate and Ethanol by successive solvent extraction by hot percolation method and aqueous extract by cold maceration. The preliminary Phytochemical screening of various extract of the plant have revealed the presence of constituents like alkaloids, flavanoids, steroids and fats & fixed oils. Quantitative estimation of alkaloids, flavonids and phenolics compounds was done. Fluoroscence analysis of powder and extract was done and it did not any fluorescent chromophore. TLC and HPTLC analysis of Ethanolic extract was carried out to identify Phytoconstituents present. In – vitro studies – DPPH assay and Hydrogen peroxide radical Scavenging assay were performed to select the most Bio – active extract. Based on this the ethanol extract was selected for In-vivo studies. Acute toxicity studies revealed that the extract was safe up to the dose of 2000 mg/kg and the LD50 values in the range of 2000 mg/kg. Hence 200 mg/kg and 400 mg/kg was selected for the study. Polycystic Ovarian disease was assessed by Letrazole induced Polycystic Ovarian disease method. The parameters studied were Body weight changes, Food intake, Vaginal exolitative cytology, Blood sugar level, Lipid profile (TC, TGs, LDL, HDL), Reproductive organ weight and Histopathological studies. Ethanolic extracts showed decrease in the elevated level of body weight, food intake, triglycerides, total cholesterol, LDL and reproductive organ weight and increase in the HDL level. All the parameters revealed the effective role of ethanolic extract in Polycystic Ovarian disease activity which was comparable to the standard. In the study of Polycystic Ovarian disease activity the Ethanolic extract shows significant activity in both In – vitro and In – vivo models. Ethanolic extract of Erythrina variegata Linn., showed many beneficial effects similar to standard drug in treating PCOS condition and inducing ovulation. These effects may be ascribed to its multiple pharmacological activities like estrogenic, antihyperlipidemic, antioxidant and hypoglycemic effects which could be useful in managing PCOS condition and prevent ovarian cell dysfunction, ovulation and thereby improving fertility. Together broad spectrum biological effects of Ethanolic extract of Erythrina variegata Linn., make it a promising drug for treating clinical and pathological abnormalities in PCOD condition. Isolation of compound using Column Chromatography was carried out to elute the individual compounds. They were further subjected for spectral analysis like IR and GC – MS. The isolated compound was proposed to be N-Hexadecanoic acid and Erucic acid. Survey of Polycystic Ovarian Disease among the girls students and working women in the age group 17-34 years was done , to collect the data and statistical measurements done. The research on all of these aspects of PCOD will inform the public about how PCOD can affect them and their loved ones.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No: 261520659 |
| Uncontrolled Keywords: | Pharmacognostical ; Phytochemical Studies ; Leaves ; Erythrina Variegata Linn ; Metabolic Symptoms ; Experimentally Induced ; Polycystic Ovarian Disease |
| Subjects: | PHARMACY > Pharmacognosy |
| Depositing User: | Ravindran C |
| Date Deposited: | 26 Mar 2018 09:00 |
| Last Modified: | 26 Mar 2018 09:00 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/6613 |
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