Shanavas, K (2017) Evaluvation of Anti-Oxidant and Anti Ulcer Activities of Ethanolic Extract of Desmostachya Bipinnata by Using Invivo and Invitro Methods. Masters thesis, Karpagam College of Pharmacy, Coimbatore.
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Abstract
INTRODUCTION: Peptic Ulcer: Peptic ulcer and other acidic symptom affect up to ten percentages of the humans with sufficient severity to prompt victims to seek medical attention. The more significant disease condition requiring medical focus is ulcer and gastro esophagealdisease1. In the US, approximately 4 million people have peptic ulcer (duodenal and gastric types), and 350 thousand new patient are diagnosed in each year, around 180 thousand peoples are admitted to hospital and treated with drugs yearly, and about five thousand patient from this case die each year as a result of ulcer condition. The lifetime of human being developing a peptic ulcer is about 10 percentages for Americans males and four percentages for female population2. Peptic ulcers is wound in the lesions in the stomach and GIT that are most often affected in younger to older adults population, but this may diagnosed in young adult life. They often appear without obvious sign and symptom, after a period of days to months of active phase of disease, it may heal with or without drug treatment. It also affect because of bacterial infections with H. Pylori. AIM AND OBJECTIVE: GIT disease against few effective drugs available for modern therapy, it produce side effect during the treatment is worse than the condition of rebound acidity. as per the literature review selected plant that cure ulcer diseases so considerable interest has developed in the examination of these numerous plants remedies which are useful for antiulcer activity. So it is necessary to find new drugs of importance in antiulcer effect with fewer side effects. Moreover it is necessary to required scientific validation traditionally using medicinal plants on various systems such as Ayurvedic Siddha Unani systems. In our study Desmostachyabipinnata plant extracts its phytochemical investigation will be a useful tool for the identification and authentication of the plant for industrial and further research purpose, which will be related to the antioxidant activity. Antioxidants, which can scavenge free radicals, have an important role in pharmacological systems. Antioxidants are emerging as prophylactic and therapeutic agents. Hence, antioxidant was also evaluated for the potent extract. And now I have under taken the study of evaluation anti-oxidant and antiulcer activity of Desmostachyabipinnata plant extracts by using invivo and invitro methods To select plant based on their ethnomedical uses and preparation of their extracts. 1. To screen phytochemical profile. 2. To screen the selected extract for antioxidant using various invitro methods 3. To screen the potent plant extract for their invitro antioxidant, 4. To evaluate invivo antiulcer activities by NSAIDs induced animal model. SUMMARY AND CONCLUSION: The present study is evaluated the antioxidant potential and antiulcer effect of AEDB. The results analysed from the present study have indicate that AEDB possesses antioxidant and antiulcer effect on aspirin induced ulcers. The preliminary phytochemical screening of whole plant extracts indicate in presence of flavonoid, alkaloid, proteins,amino acids and terpenoids, fixed oil and glycosides Pre-treatment with AEDB particularly at a dose of 400mg/kg in a single schedule and 400mg/Kg treatment significantly reduce the ulcer index value, total acidity concentration, total volume of acid release and total protein and increase value pH and glutathione content as compared with 100 mg/kg,200mg/kg and control groups. The antioxidant screening shows that it showed reducing power to DPPH radicals. But the efficiency showed that far below from Vitamin C. The concentration of the AEDB needed to scavenge 50% superoxide anion (IC50) equal to that of standard hence the plant extract have the significant antioxidant activity The antiulcer effect is screened in ethanol extract of Desmostachyabipinnata on NSAID induced anti-ulcer study. The results get from these study have been shown that ethanol extract of Desmostachyabipinnata produce antiulcer effect.In aspirin induced model, there is reduction in ulcer index, total acidity, total volume of gastric contents, total protein concentration and higher concentration of glutathione content and pH of gastric secretion they compared with control group. Famotidine used as a standard comparison agents. Famotidine used as H2 receptor blocker, is significantly reduce about 90% of basal, food induced and hormonal mediated gastric acid, which again induced by histamine, gastrin, parasympathomimetic drugs and vagal stimulation. Famotidine mediate its suppression of gastric secretion by inhibiting the histamine mediated c-AMP dependent pathway Famotidine encouraged certain type mucus analogues of gastric mucus for patients with duodenal ulcer. Total acidity responsible quantification acid is present in the gastric secretion. It has a important aggressive factor which induced the ulcer. Gastric release is maintained by vagal control and higher activity of vagus stimulation also contributes to ulcer formation. On ethanol administration, the mucosal mast cells mediate to secretion of vasoactive mediators containing histamine. Histamine is mediated to stimulate the synthesis of cyclic AMP through activation of the enzyme adenylcyclase which mediate the activation of gastric proton pump and secrete of hydrogen ions. The treatment of extracts showed reduce the total acidity of the gastric contents. Serum protein including albumin and globulin. In the peptic ulcer the total protein concentration of serum or gastric secretion are increased. This may be due to leakage of plasma protein in to the gastric secretion or serum with lower mucosal resistance/barrier of the gastric mucosal layer.After treatment with AEDB there was a significant reduction in protein concentration of gastric juice which enhancing leakage of plasma proteins. Acid volume is amount (in ml) of acid release in the gastric content release contain HCl, pepsinogen enzyme, mucus secretion, bicarbonates concentration, intrinsic factor and proteins. Amount of acid release is an important factor responsible for the production of ulcer mediated by exposure of the unprotected lumen of stomach by concentrated acids. AEDB treatment showed decrease in the acid volume of the gastric secretion. Increased pH shows a lower concentration of the hydrogen ion. The hydrogen ion is a major triggering factor responsible for the etiologic factor for ulcer and gastric damage. AEDBtreatment indicate higher concentration of pH of the gastric juices. This values directly shown the AEDB reduce possibility of ulcer and has a protective effect of surface of the gastric mucosa. In gastric ulcer tissues, Glutathione (g-glutamylcysteinylglycine, GSH) levels were found to be decreased112 . aspirin-induced genesis of free radical concentration reduces the cysteine concentration which mediated for GSH released.Values from this study responsible for depletion of gastric GSH is related with induction of gastric lesion in the rats. GSH is a tripeptide and having a superoxide radical scavenger and it protect thiol protein contents essential for release the integrity of tissue against oxidation reaction. In my present study, AEDB treatment showed increase in the glutathione content.
| Item Type: | Thesis (Masters) |
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| Additional Information: | REG.No.261526154 |
| Uncontrolled Keywords: | Anti-Oxidant ; Anti Ulcer Activities ; Ethanolic Extract ; Desmostachya Bipinnata ; Using In vivo ; Invitro Methods |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 21 Mar 2018 10:37 |
| Last Modified: | 21 Mar 2018 10:37 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/6420 |
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