Karthick, T (2017) Combined Effect of Static Magnetic Field and Metformin on Weight Loss in Overweight Rats. Masters thesis, Nandha College of Pharmacy, Erode.
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Abstract
INTRODUCTION: OBESITY:Overweight and Obesity is measured by Body Mass Index (BMI) through calculated as weight divided by height squared.Overweight was defined as a BMI ≥23 kg/m2 but <25 kg/m2 for both genders (based on the World Health Organization Asia Pacific Guidelines) with or without Abdominal Obesity (AO). Generalized Obesity (GO) was defined as a BMI ≥ 25 kg/m2 for both genders (based on the World Health Organization Asia Pacific Guidelines) with or without AO. AO was defined as a Waist Circumference (WC) ≥ 90 cm for men and ≥ 80 cm for women with or without GO. Isolated Generalized Obesity (IGO) was defined as a BMI ≥ 25 kg/m2 with waist circumference of < 90 cm in men and < 80 cm in women. Isolated Abdominal Obesity (IAO) was defined as a WC of ≥ 90 cm in men or ≥ 80 cm in women with a BMI< 25 kg/m2. Combined Obesity (CO): Individuals with both GO and AO. Non obese subjects: Individuals without either GO or AO. AIM AND OBJECTIVE:Today large number of population suffers from overweight and obesity disorder. It has been increasing due to change in life style of community on developing and developed countries. An increase in body weight is complex processes that results from sedentary life style, lack of physical exercise, particularly consumption of energy rich diets are play an important role for its development. The primary treatments used for protecting the body weight by diet control and exercise. But this is too hard to maintain and also weight regain could be possible. Though medication like Orlistat is available but these are having poor outcomes on long term weight management and are accompanied with various adverse effects. Further, progress in the study of the behavioural and biological manifestations of reducing body weight, there is no satisfactory drugs to use in clinical therapy. Preventive measures are mostly taken from alternative sources. An alternative method of treatment is getting more importance in overweight and obesity because the modern medicine does not find curative treatments. Currently there are various chemical and physical methods are available individually to the treatment of obesity but these methods can effective after long term treatment. In this research work we tried to use combination of static magnetic field with Metformin in experimental animal model as more effective and enhanced weight loss on subacute treatment. This can be obtained by synergism and it’s also safe as the strength of glucose and lipid metabolism can be reduced without compromising on decreased body weight. The present study will help to improve the biophysical knowledge among the researchers on weight loss with lesser side effects, which are affordable, non invasive and more effective with safety in the management of overweight and obesity. SUMMARY AND DISCUSSION: The present thesis entitled “Combined Effect of Static Magnetic Field and Metformin on Weight Loss In Overweight Rats” deals with the exploration of pharmacological screening of Magnetic Field with Metformin. On past two decades Magnetic field is used for treating Bone disorders and Nerve disorders. The results obtained from using magnetic field by various researchers were shown in the table 3.1 and 3.2. The SMF exposure device was fabricated by using 3-D Ansys Maxwell engineering software, 2014 version. Approval was obtained from Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) and Institutional Animal Ethics Committee (IAEC), proposal number NCP/IAEC/2016-2017- 01. Consumption of a diet rich in calories in the form of sugars and fats and its accumulation in most part of the body leads to excessive growth of adipose tissue, chronic inflammation, and metabolic perturbations. It can also lead to the development of hyperlipidemia, atherosclerosis and abnormal lipid metabolism (Onody et al., 2003). Epidemiological studies recommended that excess dietary energy intake is one of the frequent causes of obesity (Lee et. al., 2008 and Bhandari et. al., 2011). In the present study, the effects of SMF with Metformin on the alterations of glucose, liver parameter and lipid profile in high-fat diet (HFD) rats were investigated. Our earlier studies indicated that the rats fed with HFD showed significant increase in body weight, plasma glucose, insulin, and leptin, which leads to complications clinically (Sudhakara et al., 2014). The animals were grouped into Group 1 (SD Control) Group 2 (HFD control), Group 3 (HFD + Orlistat 40 mg/kg/day/po for 2 weeks), Group 4 (HFD + Static Magnetic Field 128 mT/1h/day for 2 weeks), Group 5 (HFD + Metformin 200 mg/kg/day/po for 2 weeks), Group 6 (HFD + Static Magnetic Field 128 mT/1h/day with Metformin 200 mg/kg/day/po for 2 weeks). In case of blood glucose level the drastic increase in glucose levels was due to the release of the hyperglycemic hormone (glucagon) or the inhibition of the hypoglycemic hormone (insulin) as previously shown by Lahbib, et al., 2010 and also due to conformational change of insulin was observed by Elferchichi et al., 2011, this leads to decreasing the binding capacity of insulin. The elevated glucose level in blood was reversed by treatment with Combined SMF with Metformin. This may be possibly due to the insulin like effect of SMF with Metformin on peripheral tissues, either by promoting glucose uptake and metabolism or by inhibiting hepatic gluconeogenesis. Treatment with SMF resulted in reduction in body weight indicating that SMF posess weight reducing property. The decreasing body weight might due to decrease in body fluid and protein content or other factor including hormonal changes (Lahbib, et al., 2010). In the present study, the result showed that no significant change in food and water intake and body temperature was observed on treatment with combined SMF and Metformin. Nevertheless, the body weight decrease could not be related to dehydration but probably due to hypoxia status known to alter the body weight (Amara et. al., 2006). Locomotor activity could be decreased in HFD induced rats were observed in this study. In addition to that, Increased locomotor activity were observed on treatment with combined SMF and Metformin. This may be due to decreasing in body weight with improving the functions of sciatic nerve. Previous study reported by Sekinz et al., 2006 shows alteration in sciatic nerve function on exposing SMF. CONCLUSION: This study entitled “Combined effect of Static Magnetic Field and Metformin on weight loss in overweight rats” shows a potential antiobesity activity and could protect the body against glucose, liver and lipid parameters related to obesity and its complications. Thus confirming its general use in medical practice to cure and manage body weight. There is a need for further investigation to evaluate the exact mechanism for biochemical activity in order to confirm which are responsible for antiobesity.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No.261525402 |
| Uncontrolled Keywords: | Combined Effect ; Static Magnetic Field ; Metformin ; Weight Loss ; Overweight Rats |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 21 Mar 2018 06:18 |
| Last Modified: | 21 Mar 2018 06:18 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/6410 |
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