Soundiramani, B (2016) Evaluation of In Vitro and In Vivo Antidiabetic Activity of Ethanolic Root Extract of Cassia Fistula L. on Streptozotocin Induced Diabetic Rats. Masters thesis, College of Pharmacy Madras Medical College, Chennai.
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Abstract
INTRODUCTION:The worldwide prevalence of diabetes have increased from around 60 million in 1980 to about 118 million in 1995 and are set to increase to 220 million by the year 2010 (Amoes et al.,1997). According to the International Diabetes Federation (IDF), Diabetes affects at least 285 million people worldwide and that number will be expected to reach 471 million by the year 2035 (1). Traditional use of herbal medicine is the basis and integral part of various cultures, which was developed within an ethnic group before the developed and spread of modern science. Herbal drugs constitute a major part in all the traditional systems of medicine. These have made a great contribution in maintaining human health. A majority of the world’s population still rely on herbal medicines to meet its health needs. The practice continues today because of its biomedical benefits and its place in culture beliefs in many part of world.AIM AND OBJECTIVES: 1. Successive extraction of root bark of Cassia fistula L. by Soxhlet extraction apparatus using various solvents n-hexane, ethyl acetate and ethanol. 2. Phytochemical evaluation of root bark of ethanolic extract of Cassia fistula L. 3. To determine the inhibition of alpha-amylase enzyme and glucose diffusion property of the prepared extract of the Cassia fistula L. using dialysis tube and GOD-POD kit. DISCUSSION:Diabetes is a chronic metabolic disorder affecting a major proportion of the population worldwide. A sustained reduction in hyperglycemic will decrease the risk of developing micro vascular disease and reduce their complications (51). The conventional therapies for diabetes have many shortcomings like side effects and high rate of secondary failure. On the other hand herbal extracts are expected to have similar efficacy without side effects as that of conventional drugs. The present investigation reports that antidiabetic effect of Cassia fistula on streptozotocin (STZ) induced diabetic rats (52). STZ produces oxygen radicals in the body, which cause pancreatic injury and could be responsible for increased blood sugar seen in animals (53). Pancreas has a relatively weak defense system against oxidative stress, which can be externally strengthened. Antioxidants such as N-acetylcystine and dietary antioxidant such as Vitamin C and E have shown to be beneficial in protecting the β-cells from glucose toxicity in diabetes. Medicinal plants could be considered as potential sources for providing a reasonable amount of the required elements other than diet to the patients of diabetes mellitus. Several controlled clinical trials of trace element supplements for glycemic control revealed the beneficial role for supplementation for the control and management of diabetes.Over 150 plants extracts and some of the active principle including flavonoids are known to be used for the treatment of diabetes. In our study the root of the plant extract of Cassia fistula L. indicates the presence of secondary metabolites like flavonoids, glycosides were used. The presence of these phytochemicals in high concentration account for the significant hypoglycemic effect of Cassia fistula L. Cassia fistula L. is a tropical herb/frob which has been traditionally used in the management DM (39). This present study has been undertaken to evaluate the preliminary phytochemical analysis, in vitro, acute toxicity and in vivo studies in diabetic Wistar rats. CONCLUSION: 1. From the present study we conclude that the preliminary phytochemical analysis of Cassia fistula L. indicated the presence of flavonoids, Glycosides. 2. In vitro Glucose diffusion properties of Cassia fistula L. extract was evaluated using dialysis membrane and GOD-POD kit. 3. In vitro alpha-amylase enzyme inhibition of Cassia fistula L. extract was evaluated. 4. Ethanolic extract of Cassia fistula L. was safe in dose upto 2000mg/kg by acute toxicity study and there was no toxic effects produced. 5. The ethanolic extract of Cassia fistula L. (200mg/kg and 400mg/kg) exhibited significant reduction in triglyceride & cholesterol and also reduction in the body weight in streptozotocin induced diabetic rats.6. In vivo studies of Cassia fistula L. ethanolic extract revealed the glucose lowering effect. Thus it was concluded that the ethanolic extract of Cassia fistula L. has anti-diabetic activity. 7. In this present study was exhibited significant inhibition activity of ethanolic root extract of Cassia fistula L. by using in vitro -amylase and glucose diffusion assay, so further the compound isolation, purification and characterization which is responsible for inhibiting activity, has to be done for the usage of antidiabetic agent.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg. No: 261426069 |
| Uncontrolled Keywords: | In Vitro ; In Vivo Antidiabetic Activity ; Ethanolic Root Extract ; Cassia Fistula L.; Streptozotocin Induced Diabetic Rats |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 20 Mar 2018 06:30 |
| Last Modified: | 20 Mar 2018 09:48 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/6401 |
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