Forced Degradation Studies of Pyrazinamide in Bulk and Formulation by UV, IR Spectrophotometry and UHPLC Method

Sathyaveni, G (2016) Forced Degradation Studies of Pyrazinamide in Bulk and Formulation by UV, IR Spectrophotometry and UHPLC Method. Masters thesis, College of Pharmacy, Madras Medical College, Chennai.


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INTRODUCTION: PHARMACEUTICAL ANALYSIS:Pharmaceutical analysis may be defined as a process or a sequence of process to identify and or quantify a substance or drug, the components of a Pharmaceutical solution or mixture or the determination of the structure of chemical compounds used in the formulation of Pharmaceutical product.CLASSIFICATION: Pharmaceutical analysis can be classified in the various ways All pharmaceutical analysis process can be categorized into two groups: 1. Qualitative (Identification) 2. Quantitative (Estimation) AIM AND OBJECTIVE:The aim of the study is to develop simple, novel methods for the forced degradation of Pyrazinamide in Bulk and Formulation. The quantification of Pyrazinamide have been reported and review of literature indicated that no degradation studies have been reported for pharmaceutical formulation till date The objective of the present work is develop various analytical methods such as 1. UV -SPECTROPHOTOMETRY 2. INFRA RED SPECTROPHOTOMETRY 3. UHPLC METHODS The study includes the following: 4. To perform the acid and alkali hydrolysis, oxidative degradation, thermal and Photolytic degradation. 5. Quantification of degraded product by UV and UHPLC. 6. Comparing the results of bulk and sample with standard. 7. Identification of changes in functional group present in the degraded samples by IR.SUMMMARY AND CONCLUSION: The present study involves the stress induced stability studies such as alkali and acid hydrolytic degradation, oxidative degradation, thermal and photolytic degradation. a) Degraded samples were quantified by UV and UHPLC method and the results of bulk and samples are compared with that of standard. b) Changes in the functional group in degraded samples were identified by IR. It was found that no major changes in the functional group in the inter day investigation. c) In all the methods used in this degradation study, sample undergoes greater degradation compared with that of standard. d) An important feature in this study was that sample undergoes greater hydrolytic degradation (both acid & alkali) than other degradation methods used. This is because the amide group in pyrazinamide undergoes hydrolysis to form acid. There was only mild degradation in oxidation.

Item Type: Thesis (Masters)
Additional Information: Reg. No. 261415718
Uncontrolled Keywords: Forced Degradation Studies ; Pyrazinamide ; Formulation ; UV ; IR Spectrophotometry ; UHPLC Method.
Subjects: PHARMACY > Pharmaceutical Chemistry
Depositing User: Ravindran C
Date Deposited: 19 Mar 2018 08:54
Last Modified: 21 Mar 2018 07:32

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