Design, Fabrication and Characterization of Thrombolytic Activity of Bauhinia Racemosa Extract Loaded Nanoemulsion.

Shruti, Ramesh Timane (2016) Design, Fabrication and Characterization of Thrombolytic Activity of Bauhinia Racemosa Extract Loaded Nanoemulsion. Masters thesis, R.V.S College of Pharmaceutical Sciences, Coimbatore.


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INTRODUCTION: A controlled release drug delivery system can overcome some of the problems of conventional therapy and enhance the therapeutic effect of a given drug. To obtain maximum therapeutic safety and efficacy, it becomes necessary to deliver the agent to the target tissue in the optimal amount in the right period of time there by causing little toxicity and minimal side effects. There are various approaches in delivering a therapeutic substance to the target site in a sustained controlled release fashion. One such approach is using nanoemulsion as carriers for drugs. Nanoemulsions are submicron sized emulsions that are under extensive investigations as a drug carrier for improving the delivary of therapeutic agents. Nanoemulsions are by far most advanced nanoparticle system for the systemic delivary of biologically active agents for controlled drug delivary and targeting. Nanoemulsions are thermodynamicaly stable isotropic system in which two immiscible liquids (water and oil) are mixed to form a single phase by means of an appropriate surfactant or its mix with a droplet diameter of approximately 5nm - 200nm.Because of small size nanoemulsion are transparent. AIM AND OBJECTIVE: Thrombosis is the formation of an unwanted clot within a blood vessel or heart. It is the most common abnormality of hemostasis. Thrombolytic drugs are used to lyses blood clot. Blood clot can occur in any vascular bed, however, when they occur in coronary, cerebral, or pulmonary vessels, they can be immediately life threatening. Therefore, it is important to diagnose and treat rapidly thrombosis. On this regard, formulation of a natural thrombolytic drug with advanced technique of drug delivery for a safe and effective administration is the aim of this study.The main objective of the study is to Prepare an extract of the dried aerial parts of the plant Bauhinia racemosa. Acute toxicity study of the extract. Determination of thrombolytic effect of the extract. Preparation of the nanoemulsion of the plant extract.Characterization of the nanoemulsion. Stability study of nanoemulsion. SUMMARY AND CONCLUSION: The present study was a satisfactory attempt to formulate the nanoemulsion with a view of improving the oral bioavailability and thus giving a prolonged release of drug. The main objective of the present study was to prepare an nanoemulsion of Bauhinia racemosa leaf extract for testing thrombolytic activities. No scientific study was made so far with nanoemulsion for the above activity. The leave of Bauhinia racemosa were collected and identified. They were then extracted with ethanol as solvent for 8 hours using soxhlet apparatus by hot continous extraction method. The yield was found to be 15.95% w/v. Oral acute toxicity was carried out as per OECD guidelines 423 by using rat as an animal model. A 2000 mg/kg body weight of animal dose is administered for acute toxicity study. In these studies the following parameters were observed for the evaluation of acute toxicity, which include gross behavior study, body weight analysis, hematological parameters, bio-chemical parameters, gross necropsy and histopathological studies. From the data of the acute toxicity studies it clearly demonstrates that the extract was safe up to 2000 mg/kg body weight of animal. The thrombolytic activity was carried out by in-vitro method and gave appreciable results. The extract showed activity close to that of the standard. Compatibility study was carried out by screening the components for solubility by visual observations. The nanoemulsion was prepared in 9 batches with varying oil: water ratios like 2:1, 3:1, 4:1. The batch that showed a uniform and continuous high release was selected as the optimized batch. F6 was selected as the optimized batch. The other evaluation parameters of nanoemulsion like paricle size, zeta potential, particle morphology study was done only for the optimized batch. The particle size and zeta potential reports were good.The FESEM images showed good reports but the reports would have been still better with the use of Cryo SEM technique. But it is not available in Coimbatore and it takes time to get reports from other centers with Cryo SEM. The stability study shows satisfactory results. There was no phase separation, breaking of nano emulsion or any physical changes in the nanoemulsion during the mechanical stress study. The nanoemulsion was stable at the temperature of 4±1°C, 25±1°C. Breaking of nanoemulsion was noted at 40±1°C. Therefore this study was concluded that the Bauhinia racemosa extract loaded nanoemulsion drug delivery is effective for the treatment of thrombosis.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Design; Fabrication; Characterization ; Thrombolytic Bauhinia Racemosa Extract ; Nanoemulsion.
Subjects: PHARMACY > Pharmaceutics
Depositing User: Ravindran C
Date Deposited: 14 Mar 2018 11:54
Last Modified: 14 Mar 2018 14:31

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