Study of Silver Staining of Nucleolar Organiser Regions in Malignancies of Uterine Cervix.

Narmadha, C (2013) Study of Silver Staining of Nucleolar Organiser Regions in Malignancies of Uterine Cervix. Masters thesis, Coimbatore Medical College, Coimbatore.


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In The Female Genital Tract The Cervix Is The Most Common Site Exposed To Both Bacterial And Viral Infection. It Is Also The Target For Agents Which Are Carcinogenic Leading To Invasive Cancer. According To Worldwide Cancer Statistics Cervical Carcinoma Is The Second Most Common Cancer In Women .Almost Fifty Percentage Of The Reported New Cases Every Year Have Been Proved To Be Fatal. The Declining Cervical Cancer Rates Are Due To Accurate Histologic Interpretation Of Biopsy Specimens By The Pathologist And Effective Papanicolaou (Pap) Smear Screening Programs 1. Routine Cytological Papanicolaou (Pap) Smear Screening, Early Diagnosis And Curative Therapy Has Reduced The Mortality Rate Of Cervical Cancer. Pre-Invasive Lesions Which Can Progress To Invasive Cancer Have Been Detected And Treated Earlier By Effective Screening Programs. All Features Which Are Of Diagnostic And Prognostic Significance Are Not Revealed By Routine Histopathological Techniques. This Was Due To The Difficulties In Differentiating The Malignant Aberrations From The Benign Ones Microscopically. Therefore, It Was Essential To Develop Adjunct Procedures Which Can Diagnose Malignancy With Accuracy And At The Earliest. Studies Have Showed That There Is A Definite Correlation Between Nucleolar Function, Size And The Cell Doubling Time In Human Cancer Cell Lines. This Concept 2 Has Stimulated A Revolution Of The Significance Of The Nucleus In Tumour Pathology. Cellular Proliferative Activity Can Be Assessed By A Variety Of Tests. The Method Of Counting Mitosis Can Detect Only Major Differences In The Proliferation Of Tumour Tissue .The Procedure Of Counting Mitotic Activity Is Time Consuming And Also Technically Difficult. The Disadvantage Of Flow Cytometry Is That The Tissue Is Destroyed By The Process And It Is Difficult To Evaluate The Normal Cell Population Admixed With The Cancer Cells. This Method Calculates The Percentage Of Dividing Cells By S-Phase Fraction. Markers Of Cell Proliferation Like Ki-67, DNA Polymerase, PCNA (Proliferating Cell Nuclear Antigen) Can Be Demonstrated Using Immunohistochemical Methods. But The Procedure Is Expensive And Complicated. Staining Of The Nucleolar Organising Regions By Silver Compound (Agnor) Has Gained Popularity For Its Simplicity And Easy Procedure. It Is Of Low Cost And Has Good Correlation With Other Proliferative Markers. In Proliferating Cells, Concurrent To The Increased Synthesis Of Agnor Proteins, There Is Progressive Dispersal Of Ribosomal Chromatin, Whereas In Resting Cells Ribosomal Sequences Are Located In Highly Compact Structured Chromatin. The Frequency Of Agnor Count Within Nucleus Are Significantly Higher In Malignant Cells Than In Normal, Reactive Or Benign Cell 3,4. This Is A Prospective Study To See If Quantification Of Agnor Could Help In Distinguishing Between Inflammatory, Dysplastic And Malignant Lesions Of The Cervix.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Silver Staining ; Nucleolar Organiser Regions ; Malignancies ; Uterine Cervix.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 29 Jun 2017 07:21
Last Modified: 29 Jun 2017 07:21

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