Raghu, Dhanapal (2007) Identification of Human Papilloma Virus Type 16 and 18 using Type Specific Primers in Normal Oral Mucosa, Epithelial Dysplasia and Oral Squamous Cell Carcinoma by Polymerase Chain Reaction. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
60 cases were selected. Good quality DNA for house keeping gene amplification could be obtained only for 23 cases. • 23 cases were included in our study, divided into 3 groups. • In Group I, 14 cases were histopathologically confirmed cases of OSCC of which 7 cases were well differentiated, 6 cases were moderately differentiated and 1 case was of poorly differentiated grade of OSCC. • In Group II, 5 cases were histopathologically confirmed cases of epithelial dysplasia of which 3 cases had mild dysplasia and 2 cases had moderate dysplasia. • In Group III, normal oral mucosal tissues were obtained from 4 cases. • Male to female ratio was 1:1 in Group I and Group III. In Group II all were male patients. • Mean age of cases in Group I was 58.4, Group II was 53 and in Group III mean age was 36.8. • Tissues from the three groups were amplified for the identification of human papilloma virus type 16 and 18 using type specific primers by PCR method. • HPV 18 was detected in 3 cases of Group I. • HPV 18 was not detected in Group II and Group III. • HPV 16 was not detected in Group I, Group II and Group III Our study indicates that HPV 18 has a probable role in the etiology of OSCC. However further studies in larger sample size will have to be done to establish this.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Human Papilloma Virus Type 16, Human Papilloma Virus Type 18, Primers, Normal Oral Mucosa, Epithelial Dysplasia, Oral Squamous Cell Carcinoma, Polymerase Chain Reaction. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Kambaraman B |
| Date Deposited: | 24 Jan 2018 03:53 |
| Last Modified: | 24 Jan 2018 03:53 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/5537 |
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