Immunohistochemical study of Matrix Metalloproteinase - 1 (MMP-1) expression in Oral Submucous Fibrosis

Gauri, Mishra (2007) Immunohistochemical study of Matrix Metalloproteinase - 1 (MMP-1) expression in Oral Submucous Fibrosis. Masters thesis, Ragas Dental College and Hospital, Chennai.


Download (6MB) | Preview


A total of 40 patients were included in the study, comprising of 30 patients with oral submucous fibrosis (Group 1) and 10 patients with normal buccal mucosa (Group 2). • Group 1 had a male-female ratio of 14:1, with a mean age of 35.4±9 years. In Group 2 male: female ratio of 1.5:1 was seen with 33.8±6 years to be the mean age. • All biopsies were of buccal mucosa. • All 30 cases from Group 1 were arecanut chewers. Group 2 tissue sample was obtained from subjects with no chewing habits. • The 30 cases were graded, clinically, based on the mouth opening parameter. 4 (13.3%) subjects were graded as sub-group I, 12 (40%) subjects were graded as sub-group II, 11 (36.6%) subjects graded as sub-group III and 3 (10%) subjects were graded as group IV. • All the 30 Group 1 cases, were graded histopathologically. 10 (33.3%) samples were graded as early stage, 10 (33.3%) graded as intermediate stage and 10 (33.3%) subjects were graded as advanced stage. • Both the groups were stained for Matrix Metalloproteinase-1 (MMP-1) by immunohistochemical method, using the Streptavidin HRP-Biotin labeling technique. The intensity of the cytoplasmic stain was evaluated manually using the staining intensity of the positive control (breast carcinoma) as the benchmark, and graded on a four-point scale: (-) negative staining, (+) mild staining, (++) moderate staining and (+++) intense staining. • MMP-1 expression intensity in epithelium and connective tissue was decreased in Group 1 when compared to Group 2. The results were statistically significant. (p= 0.007) • There was no statistically significant difference between the expressions of MMP-1 among different histological grades of OSF. (p= 0.38).

Item Type: Thesis (Masters)
Uncontrolled Keywords: Matrix Metalloproteinase - 1 (MMP-1), expression, Oral Submucous Fibrosis, Immunohistochemical study.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Kambaraman B
Date Deposited: 24 Jan 2018 03:53
Last Modified: 24 Jan 2018 03:53

Actions (login required)

View Item View Item