Evaluation of Microbial profile in Dental Unit Waterlines and Assessment of Antimicrobial Efficacy of Two treating Agents

Dilna, N C (2009) Evaluation of Microbial profile in Dental Unit Waterlines and Assessment of Antimicrobial Efficacy of Two treating Agents. Masters thesis, Ragas Dental College and Hospital, Chennai.

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Abstract

This study was carried out in the department of pedodontics &preventive dentistry to enumerate and identify the microorganisms present in water samples collected from dental unit waterlines of different dental speciality clinics and to find out the efficacy of two treating agents in disinfecting dental unit waterlines. Sample included 70 dental unit waterlines from different speciality dental clinics which were checked for microbial contamination. From these dental units 40 units were randomly selected and divided into two groups of 20 each. Group A, treatment was done in 20 dental units with 0.2% Chlorhexidine gluconate solution and Group B, treatment was done in 20 dental units with 10% Povidone iodine solution and the reduction in the microbial levels were assessed. Five dental units were randomly selected and checked the microbial contamination using mineral water, sterile distilled water, fresh tap water as a water source in the dental unit reservoir bottles. Five dental units were randomly selected to collect water samples from three different water outlets such as handpiece lines, air/water syringe and scaler lines and microbial contamination were checked. Also from the test group, five from each group were checked for the duration of efficacy of treating agent for one week by analyzing the water samples collected on 3, 5 and 7 day intervals. From the results we can infer that most of the identified microorganisms comprise of Gram negative and pseudomonas predominating up to 98.59% of the total isolates. Usage of disinfectants 0.2% Chlorhexidine and 10% Povidone Iodine were found to be very effective in reducing the microbial contamination and 10% Povidone iodine was found to be more efficient (97.13%) and active for a period of 3 days and gradually loosing its efficacy by 7th day. No significant difference were found in microbial contamination of water samples collected from different water outlets such as handpiece outlets, air water syringe outlets, scaler lines. According to the study results either mineral water or sterile distilled water can be preferred to fresh tap water as reservoir source in dental unit waterlines. Therefore to maintain the sterility of dental unit waterlines it is essential to have a good water source and an effective disinfectant. CONCLUSION : 1. All the test dental units showed microbial contamination which was polymicrobial in nature. 2. Most of the identified microorganisms comprise of Gram negative bacteria and Pseudomonas (98.59%) was found to be the most prevalent organism. 3. 0.2% Chlorhexidine showed 86.2% reduction in the microbial contamination. 4. 10% Povidone Iodine showed 96.14% reduction in microbial contamination. 5. 10% Povidone Iodine was found to be more effective compared to 0.2% Chlorhexidine (P<0.001). 6. Mineral water or sterile distilled water preferred to fresh tap water as a reservoir source. 7. No significant difference was found in microbial contamination of water samples collected from different water outlets such as handpiece outlets, air water syringe outlets, scaler lines (P value 0.996). 8. 0.2% Chlorhexidine can be used as an effective treating agent on daily basis. 9. 10% Povidone Iodine can be used as an effective treating agent on every 3rd day basis for microbial control.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Microbial Profile, Dental Unit Waterlines, Antimicrobial Efficacy, Two Treating Agents.
Subjects: DENTAL > Paedodontics and Preventive Dentistry
Depositing User: Kambaraman B
Date Deposited: 22 Jan 2018 11:49
Last Modified: 22 Jan 2018 11:49
URI: http://repository-tnmgrmu.ac.in/id/eprint/5522

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