Saravanaraj, M (2017) Pharmacognostical, Phytochemical and Anti-Diabetic Studies on Fruits of Adansonia Digitata Linn. Masters thesis, Madras Medical College, Chennai.
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Abstract
AIMS AND OBJECTIVES:The present study is to explore the Pharmacognostical, Phytochemical and Anti-diabetic studies on fruits of Adansonia digitata Linn. Collection and Authentication of the plant material. Establishing the pharmacognostical profile of the plant. Extraction of plant material by successive solvent extraction by increasing polarity (petroleum ether, ethyl acetate, ethanol). Phytochemical screening of the crude powder and various extracts. To evaluate the anti-diabetic activity by in vitro & in vivo methods. SUMMARY AND CONCLUSION:The project entitled “Pharmacognostical, Phytochemical and Anti-diabetic studies on fruits of Adansonia digitata Linn.," (Bombacaceae) has been achieved by the following results. Authentication plays a key role in pharmacognostical studies. The fresh fruits of Adansonia digitata Linn., was collected from the Madras Medical College Men's Hostel, Chennai and authenticated by Prof.P.Jayaraman Ph.D., Director, Institute of Herbal Botany, Plant Anatomy Research Centre. The Pharmacognostical parameters such as Macroscopy, Microscopy, Powder Microscopy, Histochemical Studies and Physicochemical Constants were studied to establish the data for characteristics feature of plant and detection of adulterants. The qualitative and quantitative analysis was carried out to identify Inorganic Elements present in the fruits. The qualitative and quantitative analysis of toxic heavy metals like Arsenic, Cadmium, Lead and Mercury were within the WHO limits and ensured the safety of the drug. In Phytochemical analysis, extraction is the first step involved. The coarse powder was extracted by Petroleum Ether, Ethyl Acetate, Ethanol by Successive Solvent Extraction by Hot Percolation Method. The preliminary phytochemical screening of various extract and powder of the plant has revealed the presence of phytoconstituents like Flavonoids, Saponins, Steroids, Tannins, Alkaloids, Glycosides And Carbohydrates. Qualitative chromatographic analysis-TLC for various extracts was carried out to identify the phytoconstituents present. In vitro studies, α-Amylase Inhibition Assay and Haemoglobin Glycosylation Inhibition Assay was performed for the selection of the Bio-active extract.Accordingly, the ethanolic extract possessed maximum anti-diabetic activity. So, it was selected for in vivo studies. The Acute toxicity studies revealed that the extract was safe up to the dose of 5000 mg/kg. Anti-diabetic activity was assessed by Streptozotocin Induced Diabetic Mellitus model. The parameters examined were Blood Glucose Level and Changes in the body weight. The Histopathological study was performed. The inference made from it were that the cells in the diabetic control group were reduced in size, damaged β-cell population and extensive necrotic changes, followed by fibrosis and atrophy. While in the group that received that the test dose showed, the absence of necrosis, fibrotic changes, increased number and size of the islets and presence of normal pancreatic cells. These were in the levels comparable with the ones that were administered the standard drug Glibenclamide. The phytochemical evaluation showed the presence of flavonoids. These compounds might be responsible for the anti-diabetic activity on the fruits of Adansonia digitata Linn. The present study revealed the Ethanolic extract has the significant anti-diabetic activity in the both In vitro and In vivo models. Further studies is required to find out the mechanism of action responsible for the anti-diabetic activity.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Pharmacognostical; Phytochemical ; Anti-Diabetic Studies ; Fruits ; Adansonia Digitata Linn |
| Subjects: | PHARMACY > Pharmacognosy |
| Depositing User: | Ravindran C |
| Date Deposited: | 08 Jan 2018 05:35 |
| Last Modified: | 08 Jan 2018 05:35 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/5374 |
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