Role of Mucin Histochemistry and Immunohistochemistry in Gastric Adenocarcinoma.

Uma, R (2012) Role of Mucin Histochemistry and Immunohistochemistry in Gastric Adenocarcinoma. Masters thesis, Thanjavur Medical College, Thanjavur.


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INTRODUCTION: Gastric cancer is the second most common type of cancer world wide. Of the gastric cancer, adenocarcinoma is the most common malignancy. It comprises over 90% of all gastric cancers. Gastric mucins are cytoprotective proteins synthesized by gastric epithelial cells. In general mucins are of two types, neutral and acid mucin. Mucin genes expression in normal stomach includes MUC1 , MUC 5AC in surface epithelium, MUC 6 in deep gastric glands. MUC 2 is not expressed in normal stomach. MUC 2 is expressed in intestinal metaplasia by goblet cells, intestinal type of gastric adenocarcinoma and mucinous gastric adenocarcinoma. MUC 2 expression is decreased in poorly differentiated gastric adenocarcinoma and variable in signet ring cell carcinoma of stomach AIM OF THE STUDY: To study the role of mucin histochemistry and immunohistochemistry in gastric adenocarcinoma. MATERIALS AND METHODS: From the period 2008 oct – 2011 sep, 50 cases of gastrectomy specimens were analysed Age, sex and site of the lesion were recorded. Subtyping of carcinoma was done. Mucin type neutral / acidic is identified by AB pH 2.5 PAS and PAS staining. Immunohistochemistry using MUC2 primary antibody was done to assess the role of its expression in various types of gastric adenocarcinoma. Results were tabulated and analysed. RESULTS: Incidence of gastric cancer among the malignancies during the period 2008 Oct – 2011 Sep is 4.4% in male – 58% and in female - 42%. Male predominate in the ratio of 3:2 with male peak incidence in the 6th decade and female peak incidence in the 5th decade. Mean age of gastric cancer – 56.7yrs(25-80). Incidence of early gastric cancer is 2% with commonest site - antropyloric region 86%.Intestinal type predominates by 61.2%. Incidence of signet ring cell carcinoma – 2%. On mucin histochemistry, acid mucin is demonstrated in 96 % of gastric cancer. Acid mucin is expressed more in poorly differentiated and mucinous adenocarcinoma type of gastric cancer. On immunohistochemistry, MUC 2 expression is more in intestinal metaplasia, >50% in mucinous adenocarcinoma, >10% in signet ring cell carcinoma, absent in intestinal type of gastric adenocarcinoma and poorly differentiated adenocarcinoma. AE1/AE3 showed diffuse and strong cytoplasmic positivity in squamous cell carcinoma.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Gastric adenocarcinoma, special stain, MUC 2 expression.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 29 Jun 2017 01:38
Last Modified: 12 Sep 2020 02:43

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