Evaluation of Hepatoprotective Activity of Ethanolic Extract of Bauhinia Tomentosa Linn. In Paracetamol & Thioacetamide-Induced Toxicity

Jeslyne, M Jeyaraj (2017) Evaluation of Hepatoprotective Activity of Ethanolic Extract of Bauhinia Tomentosa Linn. In Paracetamol & Thioacetamide-Induced Toxicity. Masters thesis, J.K.K. Nattraja College of Pharmacy, Komarapalayam.

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Abstract

AIM AND OBJECTIVE: Hepatotoxicity is a major pharmacological effect of drugs and has been an important reason for the withdrawal and banning of many drugs in the market. It is also a significant contributor of drug interactions on account of its playing a major role in the metabolism and biotransformation of drugs and other substances that enter the body. A significant amount of deaths occur due to diseases aggravated by the toxic action of therapeutic substances on the liver. There are not many measures to combat the hepatotoxicity of drugs except for reducing the dose, stopping the drug suspected to be responsible and changing the drugs substituting it with another drug of the same pharmacological action. The damage is either permanent or temporary depending on various factors like age, race, sex, underlying medical illnesses, drug interactions etc. In this study, we are looking into herbal compounds that may help offer protection from the toxic effects of paracetamol and thioacetamide on the liver of animals. Paracetamol is a commonly used NSAID. It is safe in prescribed doses but toxic in higher dosages. Paracetamol poisoning is treated currently with N-Acetyl cysteine but the treatment profile is largely unclear as we have seen in literature. In cases of poor prognosis, liver transplant could be the only option. It is expensive and the outcome may or may not be favorable. Thioacetamide is a known hepatocarcinogen. Its toxicity in animal studies is well documented. This situation warrants us to explore other remedies for treating hepatotoxicity. The plant kingdom has lots of known and unknown plants with hepatoprotective properties. Plant extracts have a wide range of medicinal actions, and throughout history, they have been used to treat many different types of diseases. In the treatment of many diseases, antioxidant therapy plays a key role, so current research is now directed towards finding naturally occurring hepatoprotectiveof plant origin. In Indian system of medicine, the plant BauhiniatomentosaLinnis being used to treat many illnesses, successfully. The medicinal properties of this plant may be due to the presence of phytochemicals like tannins, flavonoids, terpenoids and steroids. Presently it has become a source of medicine for healing human and animal diseases. Hence, in order to contribute further to the knowledge of Indian traditional medicine, and its rich history, the objective of the present study is to subject the traditionally well-known plant Bauhinia tomentosa Linn. It was decided to extract the whole plant using suitable solvents and the dried extract will be used to evaluate the possible hepatoprotective effect of Bauhinia tomentosaLinn.in albino mice. SPECIFIC OBJECTIVE: To elucidate the possible liver protective mechanism of action of Bauhinia tomentosa L. extract. To screen preliminary phytochemicals of ethanol extract of BauniniatomentosaL. � To study the effect of the extract of Bauhinia tomentosa Linn on paracetamol and thioacetamide induced hepatotoxicity in albino mice using liver enzymes as biomarkers. To study the effect of the ethanolic extract of Bauhinia tomentosa Linn. on serum bilirubin, in paracetamol and thioacetamide induced hepatotoxicity To observe suppression of inflammation and cell recovery after administration of various concentrations of the extract. To screen for anti- oxidant potential of the Bauhinia tomentosa Linn. using enzymatic (SOD, CAT, GPx) and non- enzymatic methods (GSH). To assess the free radical scavenging potential of Bauhinia tomentosa Linn. using Lipid peroxidase test (LPO) SUMMARY AND CONCLUSION: The present study was designed to evaluate the possible protective effect of ethanolic extract of Bauhinia tomentosa Linn (EEBT) against paracetamol and thioacetamide induced hepatoxicity in animals. A literature survey revealed that more studies were needed for this plant to ascertain the hepatoprotective potential. The detailed preliminary phytochemical investigations rationalized its use as a drug of therapeutic importance. Theethanolic extract of the planthas phytoconstituents like flavonoids, terpenoids, sterods, alkaloids, saponins and tannins. The hepatoprotective effect was assessed using a battery of biochemical and histopathological tests. SGOT, SGPT, ALP, LDH, ACP were some of the biochemical tests done. In vivo tests for antioxidants (SOD, CAT, GSH, LPO) were conducted on albino mice and wistar rats. In both paracetamol and thioacetamide induced hepatotoxicity, a lower dose and a high dose of extract were used and compared with the hepatoprotective activity of standard drug silymarin. Control group and an only drug group were also used. The EEBT showed marked hepatoprotective activity in lowered levels of body weight, positive effect on total bilirubin, total protein and on liver enzymes. Histological sections of liver showed that centrilobular necrosis, the pathognomonic feature of hepatotoxicity, which appeared in paracetamol-intoxicated mice, was strikingly reduced in EEBT treated mice. Furthermore, the congestion and inflammatory cell infiltration evoked by paracetamol was considerably decreased by EEBT indicating its possible antihepatotoxic action. EEBTethanolic extract has hepatoprotectiveeffects against liver toxicity induced by TAA as proven bymacroscopical, microscopical, and biochemical analyses. The effects of EEBT are comparable to that of Silymarin, the standardhepatoprotective drug. Accordingly, EEBT could beused as an effective herbal product for the prevention ofchemical-induced hepatic damage. Our results demonstrated that the progression of TAA-induced liver cirrhosis could be prevented or reduced using the ethanol extract of Bauhinia tomentosa Linn. The plant extract exerted its hepatoprotective effect by preventing the harmful cascade of events induced by TAA toxicity. In conclusion, we can say that Bauhinia tometosa Linn. has the ability to protect the liver from the damaging effects of paracetamol and thioacetamidein toxic doses and stimulation of endogenous anti-oxidant defense system. In the near future, a further study is warranted to isolate, characterize and screen the active components of Bauhinia tomentosa Linn. that have the hepatoprotective activity.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Hepatoprotective Activity ; Ethanolic Extract ; Bauhinia Tomentosa Linn. ; Paracetamol ; Thioacetamide ; Toxicity
Subjects: PHARMACY > Pharmacology
Depositing User: Ravindran C
Date Deposited: 08 Jan 2018 08:17
Last Modified: 08 Jan 2018 08:17
URI: http://repository-tnmgrmu.ac.in/id/eprint/5302

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