Devina, Dinakar (2017) Antimicrobial efficacy of different intracanal medicaments on Enterococcus faecalis and Candida albicans: An In Vitro study. Masters thesis, Sri Ramakrishna Dental College and Hospital, Coimbatore.
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Abstract
AIM : To isolate Enterococcus faecalis and Candida albicans from the plaque sample and determine their antimicrobial efficacy of the test medicaments- Nisin and Chitosan against theses microorganisms. MATERIALS AND METHODS : Plaque samples were collected from healthy human volunteers and incubated overnight. They were further streaked onto Mitis salivarius agar and Sabouraud and Dextrose Agar for the isolation of Enterococcus faecalis and Candida albicans .A total of 90 single rooted premolars were selected. Root canal preparation was done, and teeth were randomly divided into three groups. It is then further divided into five groups each according to the medicaments used (Saline, Calcium hydroxide, Nisin, Chitosan, Chlorhexidine). The canals were then sealed and all the samples were incubated at 37°C and the readings were taken on day 1,7, 14, and 21. RESULTS : The results obtained from Turbidity Testing and Colony Counting revealed that complete elimination of bacteria was not achieved in any of the experimental groups. Chitosan was most effective in causing reduction in colony counts followed by Nisin and Chlorhexidine. CONCLUSION : Nisin and Chitosan have a good antimicrobial effect against C. albicans and E. faecalis. However, the medicament that is effective against single microbe in vitro may not necessarily be effective against the same microbe in vivo because root canal system contains multiple microorganisms.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Antimicrobial efficacy, different intracanal medicaments, Enterococcus faecalis, Candida albicans, In Vitro study. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Kambaraman B |
| Date Deposited: | 04 Jan 2018 09:59 |
| Last Modified: | 04 Jan 2018 09:59 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/5215 |
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