Fabrication and Invitro Characterization of Bioactive Glass / Nano-Hydroxyapatite Reinforced Electrospun Polycaprolactone Composite Membranes for Periodontal Tissue Engineering

Vishnu, J S (2017) Fabrication and Invitro Characterization of Bioactive Glass / Nano-Hydroxyapatite Reinforced Electrospun Polycaprolactone Composite Membranes for Periodontal Tissue Engineering. Masters thesis, Sree Mookambika Institute of Dental Sciences, Kulasekharam.

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Abstract

BACKGROUND : Periodontitis is a chronic inflammatory disorder that can lead to the destruction of the periodontal tissues and ultimately tooth loss. To date, flap debridement and/or flap curettage and periodontal regenerative therapy with membranes and bone grafting materials have been employed with distinct levels of clinical success. Current resorbable and non-resorbable membranes act as a physical barrier to avoid connective and epithelial tissue downgrowth into the defect, favouring the regeneration of periodontal tissues. These conventional membranes possess many structural, mechanical, and bio-functional limitations and the “ideal” membrane for use in periodontal regenerative therapy has yet to be developed. Based on a bioactive material approach, we have hypothesized that the next-generation of guided tissue regeneration (GTR) membranes for periodontal tissue engineering will be a biologically active, spatially designed nanofibrous biomaterial that closely mimics the native extra-cellular matrix (ECM). AIM OF THE STUDY : The aim of the study was to Fabricate Bioceramic Reinforced Nanofibrous GTR membranes by the method of Electrospinning and to compare their invitro characteristics. MATERIALS AND METHODS : GTR membranes made of Polycaprolactone with a molecular weight of 80,000 reinforced with different weight concentrations of Nano Hydroxyapatite /Bioactive Glass [2%,5%,10%,15%] is fabricated by the method of Electrospinning. After fabrication, their invitro properties are evaluated. Morphology of the membranes such as Fibre diameter and Pore diameter was evaluated using SEM, Mechanical properties such as Tensile strength, Youngs modulus and Elongation at break are evaluated using Instron 3345 universal testing machine initially and after one month of Invitro degradation in PBS. Weight loss and thickness of the fabricated membranes were evaluated. For chemical and thermal stability evaluation, FTIR and TGA were performed. Percentage of viable cells were evaluated using L-929 mouse fibroblasts. RESULTS : All the Electrospun nanofibrous membranes possessed excellent Mechanical properties initially and after one month of degradation in PBS. Moreover none of the membranes found to be cytotoxic at lower concentrations and higher concentrations. On comparing the overall properties, PCL+BG2% exhibited superior cell attachment and percentage of viable cells, increased fiber and pore diameter which satisfies the ideal properties needed for GTR membranes. CONCLUSION : From the observations of the study it was concluded that the composite nanofibrous membranes prepared by Electrospinning is suitable for the use as a GTR membrane and it is a useful prototype for further development of a final membrane for clinical use.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Fabrication, Invitro Characterization, Bioactive Glass, Nano-Hydroxyapatite, Reinforced Electrospun Polycaprolactone, Composite Membranes, Periodontal Tissue Engineering.
Subjects: DENTAL > Periodontology
Depositing User: Kambaraman B
Date Deposited: 03 Jan 2018 05:05
Last Modified: 03 Jan 2018 05:05
URI: http://repository-tnmgrmu.ac.in/id/eprint/5158

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