Soumya, Omanakuttan (2016) Evaluation of Antidiabetic Potential of Whole Plant Extracts (Ethanol and Aqueous) of Coldenia Procumbens Linn. in Rats. Masters thesis, J.K.K. Nattraja College of Pharmacy, Komarapalayam.
|
Text
260416_261325216_Soumya_Omanakuttan.pdf Download (612kB) | Preview |
Abstract
AIM: Diabetes mellitus is a metabolic disorder characterized by hyperglycaemia, glycosuria, hyperlipemia, negative nitrogen balance and ketonemia. A wide-spread pathological change is thickening of capillary basement membrane, increase in vessel wall matrix and cellular proliferation resulting in vascular complications like lumen narrowing, early atherosclerosis, sclerosis of glomerular capillaries, retinopathy, neuropathy and peripheral vascular insufficiency. There is significant increase in blood glucose levels, blood cholesterol levels and blood triglycerides levels. Synthetic drugs available for the treatment of diabetes results in large number produce adverse effects after prolonged duration. There are many herbal remedies suggested for diabetes and diabetic complications. Medicinal plants form the main ingredients of the herbal remedies. Several plants have been screened for blood glucose lowering activities on experimental animals. Individual plants as well as herbal formulations were reported as hypoglycaemic and antidiabetic agents. However, very few plants have been studied in depth for the blood glucose lowering effect and mechanism of action for development as an antidiabetic agent. The plants such as Allium cepa, Allium sativum, Syzygium cumini, Momordica charantia, Gymnema sylvestre, Pterocarpus marsupium, Coccinia indica, Trigonella foenum graceum, Psidium guajava and Aegle marmelos have attracted more attention of scientists in recent years. Coldenia procumbens has been used for treatment of diabetes in folklore medicine. About 10- 15 leaves are boiled in cup of water and used for diabetes mellitus. Very little work has been reported on phytochemical Pharmacological screening of Coldenia procumbens. Hence, it is therefore decided to investigate the antidiabetic potential of leaves of Coldenia procumbens. Qualitative chemical tests are proposed to be carried out in the 90% ethanol extract, 50% ethanol extract and aqueous extract in order to find out the chemical constituents. Antidiabetic studies are proposed to be carried out using rats in three different models. The models proposed are: Fasting glucose model Oral glucose tolerance test (OGTT) in rats and 3) Streptozotocin induced diabetes model in rats. Blood glucose levels, total cholesterol levels, and triglyceride levels would be estimated to asses the antidiabetic activity of the selected plant extracts. SUMMARY AND CONCLUSION: The present study was designed to evaluate the antidiabetic potential of Coldenia procumbens. Preliminary chemical studies included qualitative chemical tests. Antidiabetic evaluation of Coldenia procumbens was done using 18 hr fasting glucose model, oral glucose tolerance test and streptozotocin induced diabetes model. Qualitative chemical tests revealed the presence of carbohydrates, flavonoids, steroids, tannins and phenolic compounds in 50% ethanol extract and 90% ethanol extract and presence of carbohydrates, proteins and amino acids, flavonoids, steroids, tannins and phenolic compounds in aqueous extract. Antidiabetic studies on 50% ethanol extract, 90% ethanol extract and aqueous extract of Coldenia procumbens was carried out using fasting glucose model, oral glucose tolerance test and streptozotocin induced diabetes model. 90% ethanol extract, 50% ethanol extract and aqueous extracts in dose of 250 mg/kg were devoid of hypoglycemic activity in fasting glucose model. 90% ethanol extract and 50% ethanol extract in the dose of 250 mg/kg showed antihyperglycaemic activity in oral glucose tolerance test. 50% ethanol extract is more effective than 90% ethanol extract in oral glucose tolerance test. 90% Ethanol extract and 50% Ethanol extract in dose of 250mg/kg showed antidiabetic activity in streptozotocin induced diabetic rats. 50% ethanol extract is more effective than 90% ethanol extract in the dose of 250 mg/kg in streptozotocin induced diabetic rats. 50% ethanol extract of Coldenia procumbens showed significant reduction in triglycerides levels in streptozotocin induced diabetic rats. Effect on blood glucose level in fasting glucose model: In the single dose treatment at 250mg/kg p.o., none of the three extracts of Coldenia procumbens showed hypoglycemic activity in fasting glucose model which indicates that these extracts were devoid of insulin secreting or insulin like activity. Effect on blood glucose level in oral glucose tolerance test: In oral glucose tolerance test (OGTT) the lowering of blood glucose was observed at 30 and 60min. post glucose administration when compared to control. Effect on blood glucose level in streptozotocin induced diabetes model: In streptozotocin induced diabetes model, both the extracts i.e. 50% ethanol extract and 90% ethanol extract of Coldenia procumbens exhibited varied degree of reversal of diabetic condition with maximum effect in 50% ethanol extract when compared to the streptozotocin control. In the group treated with 50% ethanol extract, the blood glucose level significantly reduced to 146.85 ± 14.18 mg/dl on day 28 as compared to that of 300.10 ± 23.03 mg/dl in streptozotocin control. The 50% ethanol extract may be subjected fractionation in order to enrich an active fraction and thereby the phytochemical responsible for this antidiabetic activity may be identified, isolated and characterized. It may be concluded that the leaves of Coldenia procumbens possess antidabetic activity in animal models.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Antidiabetic Potential ; Plant Extracts ; Ethanol ; Aqueous ; Coldenia Procumbens Linn ; Rats |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 05 Jan 2018 05:39 |
| Last Modified: | 05 Jan 2018 05:39 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/4945 |
Actions (login required)
 |
View Item |
