Vijay, S (2017) Evaluation on Anti-Diabetic Effect of Ethanolic Extract of Whole Plant of Nymphaea Pubescens on Streptozotocin Induced Diabetes in Wistar Rats. Masters thesis, C.L.Baid Metha College of Pharmacy, Chennai.
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Abstract
The present study were Management of Diabetes mellitus is a global problem, successful treatment is very important for preventing or at least delaying the onset of long term diabetic complications like diabetic neuropathy, nephropathy, retinopathy, erectile dysfunction, hypertension and injury caused by ischaemic and reperfusion. Through nature in the form of herbal medicines or drugs with very minimal adverse effects are preferred when compared to the available synthetic drugs to treat such chronic disease and disorders. Herbal drugs as therapeutic agents are a nature’s boon when compared to the severe adverse effects of the allopathic medical practice for diabetes, despite the fact that the search for a complete and permanent cure for the disease is being pursued uncompromisingly by eluding physicians and researchers. These herbal remedies which exemplifies the process of symbiosis still remains unfamiliar up to data technical advances, which has fashioned a marvelous scope for folk lore or traditional medicines .It is supposed that the tradition medicines used for the treatment of diabetes mellitus satisfy the sequence of complication of the disease. Even though, the traditional medicinal plants are used to cure the disease from human origin, scientific validation of such medicinal plants are necessary and also a scientific research to prove its pharmacological and therapeutic efficacy is became vital. Nymphaea pubescens willd is having the anti-diabetic activity. As per the literature review still no anti-diabetic activity has been reported on this whole plant. Hence, this study has been taken to explore the anti-diabetic potential of Nymphaea pubescens willd on streptozotocin induced diabetes in wistar albino rats.DISCUSSION AND CONCLUSION:The ethanolic extract from whole plant of Nymphaea Pubescens were subjected to preliminary phytochemical analysis which shown presence of flavonoids, alkaloids, tannins, proteins, steroids and phenol with absence of saponins and anthroquinones. Acute oral toxicity studies of EENP did not produce any mortality or signs of toxicity at the dose of 2000 mg/kg b.w/p.o, in experimental rats. The anti-hyperglycemic effects of the plants is due to their ability to restore the function of pancreatic tissues by causing an increase in insulin output or inhibit the intestinal absorption of glucose or to the facilitation of metabolites in insulin dependent processes. Hence, treatment with herbal drugs has an effect on protecting b cells and smoothing out fluctuation in glucose levels72 The present study evalulation of anti-diabetic activity of whole plant of Nymphaea pubescens STZ induced diabetic rats. Experimental induction of hyperglycemia with STZ is associated with the characteristic loss of body weight which is due to loss or degradation of structural proteins it leads to increased muscle wasting and due to loss of tissue protein, as the structural proteins are known to contribute to body weight. Diabetic rats treated with glibenclamide and EENP showed increased body weight when compared to untreated diabetic animals. It may be due to increased insulin secretion and glycemic control of EENP. Reduced glucose transport or absorption from the gut, extra pancreatic action probably by stimulation of glucose utilization in peripheral tissues, increase in glycogenic or glycolytic enzyme activities in peripheral tissues, decrease in the secretion of counter-regulatory hormones like glucagon, growth hormones are the possible mechanisms involved with suppressing blood glucose levels. The glibenclamide, stimulating insulin secretion from pancreatic β cells principally by inhibiting ATP sensitive KATP channels in the plasma membrane and decreases the blood glucose level73. Blood glucose level decreased significantly in glibenclamide and EENP treated diabetic rats and the histopathology of pancreas showed normal islets in pancreas with normal anatomy compared with normal rats which may be due to the anti-diabetic activity. Hyperglycaemia is accompanied with dyslipidemia under normal circumstances; insulin activates the enzyme lipoprotein lipase, which hydrolyses triglycerides. However, in diabetic state lipoprotein lipase is not activated due to insulin deficiency, resulting in hyper triglyceridemia, and insulin deficiency is also associated with hyper cholesterolemia due to metabolic abnormalities. The dyslipidemia is characterized by increase in TC, LDL, VLDL, TG and fall in HDL which is observed in STZ induced diabetic rats74. The diabetic rats treated with glibenclamide and EENP showed reduced severity of dyslipidemia with decrease in TC, LDL, VLDL, TG and increase in HDL. Both SGOT and SGPT enzyme levels get elevated during liver damage which is more in diabetic rats75. The diabetic rats treated with glibenclamide and EENP reduced the SGOT and SGPT level. The liver histopathology of STZ induced diabetic rats showed centrilobular necrosis accompanied by fatty changes and ballooning degeneration in the hepatocytes treatment with EENP reversed in diabetic rats treated with glibenclamide and EENP which indicates that the liver damage is reduced in EENP treated group. The diabetic hyperglycaemia induces elevation of the serum levels of creatinine which are significant markers of renal dysfunction, The treatment of EENP in rats showed marked decrease in serum creatinine levels in diabetic animals.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Anti-Diabetic Effect ; Ethanolic Extract ; Nymphaea Pubescens ; Streptozotocin ; Induced Diabetes ; Wistar Rats. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 26 Dec 2017 02:02 |
| Last Modified: | 26 Dec 2017 02:09 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/4811 |
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