Timai, Passah (2017) Anti-Inflammatory and Analgesic Activity of Ethanolic Extract of Kaempferia Rotunda Rhizome in Rats. Masters thesis, RVS College of Pharmaceutical Sciences, Coimbatore.
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Abstract
AIM & OBJECTIVE: Inflammation is a pathophysiological response of living tissue to injury that leads to local accumulation of plasmatic fluid and blood cells. Pain has an unpleasant sensory and emotional experience associated with actual or potential tissue damage. Many drugs that are currently used for the management of pain are opioids or non-opioids and that for inflammatory conditions are non-steroidal anti-inflammatory drugs (NSAIDs) and corticosteroids.Asmost of these chemically induced drugs produce some of the side effect.Thus in this study, I have selected the rhizome of Kaempferia rotunda as a potent anti-inflammatory and analgesic evaluation and less side effectwhich is commonly used traditional medicine. The rhizome is ground into paste and applied externally for the treatment of sprains for local use. Hence present study was undertaken to evaluate the anti-inflammatory and analgesic activity of Kaempferia rotunda in Wistar albino rats using ethanol extract. DISCUSSION: Phytochemical screening: Qualitative Phytochemical screening and ethno botanical survey on Kaempferia rotunda are evalauted the presence of certain phyto constitutions such alkaloids, flavanoids, protein, steroids,terpenoids, tannins . The phytochemical constituents such as, flavonoids, alkaloids may be linked to the analgesic and anti-inflammatory activity. Acute toxicity studies To check the safety profile of the Kaempferia rotundaethanolic extract was subjected to the acute toxicity study which confirmed the absence of any toxicity or mortality at the higher dose of 2000mg/kg. Thus, the ethanolic extract of Kaemferia rotunda can be classified as a safe drug category according to the Global harmonized Classification System quoted in the OECD guidelines 1996. Based on the Toxicity studies 50mg/kg is used as a dose of extract and middle dose 200mg/kg and higher dose as 2000mg/kg used for sub-acute toxicity studies. Anti-inflammatory and analgesic activity Carrageenan induced paw odema was widely used to screen anti-inflammatory drugs. Screening of anti-inflammatory activity of ethanolic extract of Kaempferia rotunda was done by Carrageenan induced paw odema in rat model. While screening of analgesic activity is done by using Eddy’s hot plate in swiss albino mice model and Tail immersion method by Wistarrat model. The ethanolic extracts of Kaempferia rotunda in varying doses such as (300, 600 and 900mg/kg body weight) was given orally.From the table showed that ethanolic extracts of Kaempferia rotundashowed significant reduction in the paw odema at 4hr(600mg/kg 55.35 900mg/kg-66.30) is quite similar when compared to the group treated with standard drug diclofenac sodium (10mg/kg-69.28). Carrageenan induced paw odema in rat model is biphasic in nature.Earlier phase (1-2hr) of the carrageenan model is mainly mediated by histamine, serotonin and increased production of prostaglandins. Then the later phase is sustained by the prostaglandin release mediated by Bradykininleukotrienes, Polymorphonuclear cells . The highest percentage of inhibition was found with the dose of 900mg/kgie, 66.30. The central analgesic activity of the test drug was studied against thermal stimuli using the hot plate test and tail immersion method. In case of Eddys hot plate and Tail immersion methods the ethanolic extracts of Kaempferia rotunda (200mg/kg,400mg/kg) increase the stress tolerance capacity of animals.In Tail immersion test the time taken to reach the peak analgesic activity +30 and 1hr which is similar to that of standard drug and in Eddys hot plate the time take to reach the peak analgesic activity at +60 minute which is lower to the standard drug +30 minute. From the results it is shown that the ethanolic extracts of Kaemferia rotundapossess significant analgesic andanti-inflammatory activity. CONCLUSION: The presented study is an attempt to investigate the Anti-inflammatory and Analgesic activity of ethanolic extract of Kaempferia rotunda rhizomes. The Phytochemical screening showed the presence of Alkaloids, Terpenoids, Steroids, Tannins, saponins, flavonoids and proteins which is responsible for Analgesic and Anti-inflammatory activity. The finding of the preseninvestigation suggests the Ethanolic extract ofKaempferia rotunda has potential for its evaluation as an Analgesic and Anti-inflammatory activity. Toxicity studies do not produce any toxic symptoms on Acute toxicity study. In conclusion the Ethanolic extract of Kaempferia rotunda are safe and can be used as Analgesic and Anti-inflammatory without any harmful effects. Further studies are required to confirm the exact mechanism behind the Analgesic and Anti-inflammatory activity of Kaempferia rotundaethanolic rhizomes extract.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Anti-Inflammatory ; Analgesic Activity ; Ethanolic Extract ; Kaempferia Rotunda Rhizome ; Rats. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 26 Dec 2017 02:24 |
| Last Modified: | 26 Dec 2017 02:24 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/4770 |
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