Kavya, V (2017) Evaluation of Ethanolic Extract of Ottelia Alismoides (L.) Pers on the Pain Threshold Response in STZ Induced Diabetic Neuropathic Pain Model in Rats. Masters thesis, The Erode College of Pharmacy and Research Institute, Erode.
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Abstract
Aim: The present investigation was aimed to screen the Diabetic neuropathy activity of Ottelia alismoides (L.) PERS on Streptozotocin induced Diabetic Wistar albino rats. Objectives: The objective of the present study to: Find out the phytochemical constituents present in the ethanolic extract of the whole plant of Ottelia alismoides (L.) PERS Acute toxicity studies Evaluation of anti-diabetic activity Blood glucose level, Body Weight, Organ Weight (Liver and Kidney), Estimation of Antioxidant parameters Superoxide dismutase level (SOD), Catalase level (CAT), Reduced glutathione (GSH), Glutathione peroxidase (GPx). Other parameter: Na+ ,K+, ATPase acivity Estimation of Oxidative stress parameters: Lipid peroxidation (LPO), Estimation of Behavioral assessment parameters: Hot plate (Thermal Hyperalgesia), Cold plate (cold allodynia), Tail immersion (Thermal hyperalgesia), Tail clip (Mechanical hyperalgesia), Tail Flick (Thermal hyperalgesia), Formalin (Chemical hyperalgesia) Histopathological studies of sciatic nerve Statistical analysis SUMMARY AND CONCLUSION: The present study was to investigate the Neuroprotective effect of EEOA in experimentally induced diabetic neuropathy in rats. The phytochemical investigation reveals that the presence of tannins, phenols & flavanoids are proved to be effective in diabetic treatment. Diabetogenic effect of STZ is more reproducible and the metabolic changes are much closer to those existing in human diabetes. Hence STZ is considered the drug of choice for the induction of experimental diabetes. The Blood glucose level significantly decreased with treatment of EEOA proves that it has anti-diabetic activity. The study shows marked inhibit in oxidative stress by enhancing reduced activity of antioxidant enzyme SOD, CAT, GSH, GPx, Na+, K+, ATPase & markedly elevate the level of LPO in liver compare to untreated diabetic control animals, these findings further strengthen beneficial effect of Ottelia alismoides as it showed production against STZ induced diabetic neuropathic pain in rats. After 4th week, diabetic control has shown signs of neuropathy while treated group has showed significant improvement in their condition. From this results, it is evident that EEOA has beneficial effect in protective against STZ- induced diabetic neuropathy in rats. Histopathological assessment also showed that the damage caused by STZ to the sciatic nerve is also markedly reduced by the administration of Ottelia alismoides. Finally these findings suggest that Ottelia alismoides treatment must be beneficial to treat pain in diabetic animals.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Ethanolic Extract ; Ottelia Alismoides (L.) Pers ; Pain Threshold Response ; STZ Induced Diabetic Neuropathic Pain Model in Rats. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 26 Dec 2017 02:25 |
| Last Modified: | 26 Dec 2017 02:25 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/4767 |
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