Evaluation and Comparative Analysis of Newer Compound Fixatives

Muthuselvi, S (2017) Evaluation and Comparative Analysis of Newer Compound Fixatives. Masters thesis, Tirunelveli Medical College, Tirunelveli.


Download (1MB) | Preview

Download (7kB) | Preview


BACKGROUND : Fixation is the crucial step in the practice of diagnostic pathology. Formalin has been used as a general fixative for more than one century. The IARC (International Agency for Research on Cancer) reported that there is a causal association between formalin exposure and leukemia and nasopharyngeal carcinoma. Hence it is necessary to find a alternative fixative to replace formalin in pathology laboratory. METHODS : 100 specimens were fixed in 3 different minimal formalin containing compound fixatives at 10, 8 and 7 hours. Compound fixatives were prepared with varying concentrations of formalin, ethanol, glycerin and hypotonic saline. The processed specimens were analysed for fixation artifacts, staining characteristics and cytoarchitectural features and compared with conventional formalin fixation. RESULTS : Cytoarchitectural features (nucleus, cytoplasm and architecture) were preserved as well as conventional formalin fixation in both fixative 1and 2 at 10 & 8 hours. 7 hours fixation resulted in inadequate fixation. Fixative 3 resulted in poor preservation of tissues in all three fixation times. Fixative 2 found to evaporate less formaldehyde vapor than 10% buffered formalin and fixation time reduced upto 8 hours. CONCLUSION : As formaldehyde is a group 1 human carcinogen, it should be replaced by less toxic fixatives in histopathology laboratories. The present study highlights that minimal formalin containing compound fixatives can be easily prepared in the laboratory and their effectiveness in routine histopathology examination is comparable with conventional formalin fixation.

Item Type: Thesis (Masters)
Uncontrolled Keywords: formalin toxicity ; compound fixatives ; shrinkage artefacts.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 22 Dec 2017 17:04
Last Modified: 22 Dec 2017 17:04
URI: http://repository-tnmgrmu.ac.in/id/eprint/4726

Actions (login required)

View Item View Item