A Study on Significance of p16INK4a and Ki67 Proteins in Cervical Biopsies

Shivapriya, R (2017) A Study on Significance of p16INK4a and Ki67 Proteins in Cervical Biopsies. Masters thesis, Coimbatore Medical College, Coimbatore.


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BACKGROUND : Cervical cancer is a leading cause of cancer deaths among women in India. Human papillomavirus is a prime cause of cervical cancer. E6 and E7 viral DNA by binding p53 and pRB , increases the expression of ki67 and p16INK4a proteins. AIM : This study evaluates the expression of p16INK4a and ki67 proteins in cervical biopsies. MATERIALS AND METHODS : Forty cases including chronic nonspecific cervicitis -14, LSIL-6, HSIL-6 and carcinoma -14 were subjected to immunohistochemical study with p16INK4a and ki67. p16ink4a scoring were given as 0,1,2 and 3 when < 5%,6 - 25%,26 - 50% and > 50% cells showed nuclear and cytoplasmic positivity respectively. For ki67 in benign and premalignant lesions , no staining, lower 1/3rd , lower 2/3rd and > 2/3rd of epithelium with nuclear positivity scored as 0,1,2 and 3 and in carcinoma, 0%,< 30%,30 to 50% and >50% cells positivity as 0,1,2 and 3 respectively. RESULTS : All benign cases were negative, 16.7% LSIL(score-1),100% HSIL and carcinoma (score 2and 3) showed p16INK4a positivity, showing graded increase in score from benign to malignant lesions (p value <0.05). All benign cases ( score1) , 100% LSIL(score-2),100% HSIL and carcinoma(score 2 and 3) showed ki67 positivity ,showing increase with proliferation (p value <0.05). p16INK4a and ki67 are positively correlated (p value=0.001) CONCLUSION : p16INK4a and ki67 are useful biomarkers in cancer diagnosis and prognosis and distinction of reactive lesions from LSIL and HSIL.

Item Type: Thesis (Masters)
Uncontrolled Keywords: cervical cancer ; p16ink4a ; ki67.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 22 Dec 2017 16:49
Last Modified: 22 Dec 2017 16:49
URI: http://repository-tnmgrmu.ac.in/id/eprint/4724

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