Archana, Teresa (2014) Clinical and biochemical studies on senile cataract. Masters thesis, Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirapalli.
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Abstract
AIM: To determine the frequency of occurrence of different types of senile cataract and assess the possible putative correlations with lenticular protein profiles and to compare visual outcomes and factors influencing them, in patients undergoing small incision cataract surgery (SICS) and phacoemulsification cataract surgery at a tertiary eye care hospital in India. PATIENTS AND METHODS: 520 patients-130 in the SICS group and 390 in the phaco group were enrolled in the study. The putative factors influencing the outcome of surgery, such as gender, the presurgical presence of systemic co-morbid conditions and of ocular conditions, and the degree of nuclear sclerosis of the cataractous lens, was ascertained. Differences in degree of visual improvement between the two surgical groups were sought .An attempt was made to study the human lens proteomics , by subjecting varying grades of nuclear cataract to sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE) and 2DE analytical techniques, and the results obtained, were analysed. Results: Analysis of lenticular profiles revealed that NC3 was the most common cataract among males and in the SICS group, whereas NC4 was more common among females and in the phaco group. There was no difference in postoperative mean visual acuity between SICS and phaco in males, although such a difference existed in females with better visual acuity in the phaco group. Proteomic studies showed that the cataractous lens samples showed a statistically significant reduction in band intensity on SDS-PAGE and subsequently done two dimensional gel electrophoresis (2-DE) and MALDITOF analysis revealed varying levels of alpha-crystallins in the cataractous lens Conclusion: Both SICS and phacoemulsification yield satisfactorily similar visual outcomes and there are no other obvious factors such as grade of cataract, associated ocular and systemic comorbid conditions, that would favour choosing one procedure rather than the other. Additional biochemical studies on various grades of human cataract may help in identifying factors triggering cataractogenesis
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | senile cataract; Clinical studies; biochemical studies; small incision cataract surgery; SICS |
| Subjects: | MEDICAL > Ophthalmology |
| Depositing User: | Devi S |
| Date Deposited: | 13 Dec 2017 07:25 |
| Last Modified: | 13 Dec 2017 07:25 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/4345 |
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