A Comparative Study on Serum Levels of Nitric Oxide before and After Initial Periodontal Therapy in Healthy and Type 2 Diabetes Mellitus Patients with Chronic Periodontitis.

Gayathri, S (2013) A Comparative Study on Serum Levels of Nitric Oxide before and After Initial Periodontal Therapy in Healthy and Type 2 Diabetes Mellitus Patients with Chronic Periodontitis. Masters thesis, Sree Mookambika Institute of Dental Sciences, Kulasekharam.

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Abstract

BACKGROUND: Abnormal nitric oxide (NO) synthesis has been implicated in the pathogenesis of both periodontal disease and diabetes mellitus. Nitric oxide (NO) is a highly reactive, free radical which has complex roles in both health and disease. Nitric oxide (NO) is both harmful and beneficial to the general pathophysiology of tissues. When iNOS is released simultaneously with superoxide it forms the reactive nitrogen species peroxynitrite anion. This peroxynitrite is believed to be responsible for many of the cytotoxic effects. AIM OF THE STUDY: To evaluate serum levels of nitric oxide in healthy, chronic periodontitis and chronic periodontitis patients with type 2 diabetes mellitus before and after initial periodontal therapy. OBJECTIVES OF THE STUDY: 1) To find out the effect of initial periodontal therapy on serum levels of nitric oxide in healthy, chronic periodontitis and chronic periodontitis patients with type 2 diabetes mellitus 2) To evaluate whether nitric oxide is an indicator of the inflammatory status of periodontium MATERIALS AND METHODS: This was an interventional study including a total of 90 (30 healthy controls, 30 chronic periodontitis and 30 chronic periodontitis with type 2 diabetes mellitus) subjects. Clinical parameters (OHI-S, GI, PPD and CAL) were recorded at baseline and 4 weeks after initial periodontal therapy. Initial periodontal therapy included oral hygiene instructions and full mouth subgingival scaling. 5ml of venous blood sample was collected from each subject at baseline and four weeks after initial periodontal therapy. Serum nitric oxide (NO) levels were assayed by Griess colorimetric reaction. Optical density was measured at 545 nm using a spectrophotometer. RESULTS: The periodontal parameters- Oral Hygiene Index-Simplified(OHI-S), Gingival Index (GI)- in all the three groups showed a marked reduction at the end of four weeks following initial periodontal therapy from its baseline values. No pronounced reduction was observed in the mean values of probing pocket depth (PPD) and clinical attachment level (CAL) from its baseline values at the end of four weeks. However there was a statistically significant reduction in the serum levels of nitric oxide at the end of four weeks in all the three groups. Simultaneous comparison of pre treatment and post treatment periodontal parameters and serum levels of nitric oxide between the three groups showed that the mean values of group II and group III were higher when compared to group I. However comparison of the pretreatment mean value of Gingival Index (GI) alone was statistically significant between group II and group III. Similarly the post treatment mean values of Oral Hygiene Index-Simplified(OHI-S), Gingival Index (GI) and serum levels of nitric oxide were statistically significant between group II and group III. CONCLUSION: Serum levels of NO are elevated in individuals with chronic periodontitis and chronic periodontitis with type 2diabetes mellitus. This study provides evidence that initial periodontal therapy contributes to reduction in serum levels of NO in these patients. Larger controlled trials are needed to confirm these findings.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Serum Levels ; Nitric Oxide ; before and After ; Initial Periodontal Therapy ; Healthy ; Type 2 Diabetes Mellitus ; Patients ; Chronic Periodontitis ; Comparative Study.
Subjects: DENTAL > Periodontology
Depositing User: Subramani R
Date Deposited: 13 Nov 2017 18:14
Last Modified: 13 Nov 2017 18:14
URI: http://repository-tnmgrmu.ac.in/id/eprint/3942

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