Comparison of Salivary and Serum Levels of TNF Alpha and IL-4 in Chronic Periodontitis and Periodontal Health.

Teenu, Abraham (2013) Comparison of Salivary and Serum Levels of TNF Alpha and IL-4 in Chronic Periodontitis and Periodontal Health. Masters thesis, Ragas Dental College and Hospital, Chennai.


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BACKGROUND: Peridontitis is a chronic inflammatory disease that results in the destruction of the tooth supporting apparatus. It is believed to be mediated by the exaggerated host response that results from a disharmony between the pro inflammatory cytokine and anti inflammatory cytokine released during the inflammatory process. Recent advancements have enabled to detect the levels of proteins in oralfluids that may act as a bio marker for periodontal disease. This study was therefore conducted to assess the levels of salivary cytokines in patients with advanced periodontitis and healthy subjects. AIM: To analysis the levels of pro inflammatory cytokine TNF alpha and anti inflammatory cytokine IL-4 in saliva and serum and correlate it with the level of systemic cytokine hs-CRP in saliva and serum of chronic periodontitis patients and healthy subjects. MATERIALS AND METHODS: 30 patients were included in the study. The study group was segregated as health group and chronic periodontitis group. Serum and saliva were collected from both the groups and the levels of TNF alpha and IL-4 was detected using ELISA. hs-CRP levels were detected using immunoturbidity method. Statistical analysis was done using independent sample student T test and Pearson correlation test. RESULTS: The levels of salivary TNF alpha in disease showed a statistical significance (P=0.035, P<0.05). There was a significant correlation between the salivary levels of hs-CRP and TNF alpha in serum (p<0.001). However the levels of both cytokine in saliva and serum were not significant. CONCLUSION: Chronic periodontitis may be mediated by the Th2 response and TNF alpha may be used as a bio marker for periodontal diseases as its level showed a significant increase in periodontitis. The nonspecific serum marker for systemic inflammation hs-CRP may be well correlated to the pro inflammatory cytokine TNF alpha than the anti-inflammatory marker IL-4.

Item Type: Thesis (Masters)
Uncontrolled Keywords: TNF alpha ; IL-4 ; pro inflammatory cytokine ; chronic periodontitis ; anti-inflammatory cytokine ; saliva.
Subjects: DENTAL > Periodontology
Depositing User: Subramani R
Date Deposited: 13 Nov 2017 18:05
Last Modified: 13 Nov 2017 18:05

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