Saumya, John (2013) Expression of B7-H3 in Healthy and Diseased Gingival Samples: An Immunohistochemical Study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: T cells have been shown to play a role in etiopathogenesis of periodontal disease. B7-H3, a costimulatory molecule is found to be associated with regulation of T cell function in other chronic inflammatory diseases. MATERIALS & METHODS: Gingival samples were taken from 2 groups of patients (group A - periodontal health and group B- periodontal disease). These were paraffinised and processed to carry out immunostaining to identify B7-H3 expression. The slides were then examined under light microscope to assess the positive staining in epithelium and connective tissue. The intensity of positive staining in epithelium and the number of positive cells in the connective tissue were evaluated. Statistical analysis was done using kappa analysis and independent ‘t’ test. RESULTS: Kappa analysis revealed good inter-examiner agreement for both the groups (group A - 0.718; group B - 0.797). Intensity of staining in the epithelium ranged from intense to moderate for both the groups. In the connective tissue there was a statistically insignificant decrease (p value- 0.415) in the number of positive cells from group A (mean labeling index- 50.28±26.09) to group B (mean labeling index- 41.37±25.29). CONCLUSION: B7-H3 expression demonstrated a statistically insignificant decrease in disease as compared to health.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | B7-H3 ; Periodontitis ; Immunohistochemistry. |
| Subjects: | DENTAL > Periodontology |
| Depositing User: | Subramani R |
| Date Deposited: | 13 Nov 2017 18:00 |
| Last Modified: | 13 Nov 2017 18:00 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3940 |
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