An In Vitro Evaluation of Antimicrobial Property and Smear Layer Removal Efficiency of Fragaria Ananassa Extract on Root Canal Dentin

Venkatesh, B (2013) An In Vitro Evaluation of Antimicrobial Property and Smear Layer Removal Efficiency of Fragaria Ananassa Extract on Root Canal Dentin. Masters thesis, Ragas Dental College and Hospital, Chennai.

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Abstract

BACKGROUND: Phytomedication a possible alternative in contemporary endodontics. AIM: The aim of this study was to investigate the minimum inhibitory concentration of Fragaria Ananassa against E.Faecalis and to evaluate the MIC of F.A in removing smear layer along the length of the root dentin. METHODOLOGY: Crude F.A extract was serially diluted in Neat, 1:2, 1:4 and 1:8 concentration. MIC was tested against standard strain of E.Faecalis in blood Agar suspended in BHI broth. 40 extracted human mandibular premolar tooth was selected. Divided into 4 groups. (10 tooth per group). Group 1 – 50% F.A., Group 2 – 17% EDTA, Group 3 – 5.25% Naocl, Group 4 – saline (control). Irrigation was performed with respective irrigants (10 ml) for every change of file for one minute (S1 – F3 protaper). Ultrasonic irrigation was done for one minute for every change of file. 10ml of distilled water was used as a final irrigant for one minute. Tooth was split and examining under SEM. RESULTS: Group 1 showed superior smear layer removal along Apical, Middle, Coronal portion. Group 2’s ability to remove smear layer in the apical portion was less than Group 1 whereas in middle and coronal portion it was comparable with group 1. Group 3’s ability to remove smear layer was less than Group 1 and Group 2. Group 4 was least effective. CONCLUSION: The MIC of F.A against E.Faecalis was 50%. 50% F.A was superior to 17% EDTA and 5.25% Naoacl in removing smear layer along the entire length of the canal.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Irrigation ; Smear layer ; Fragaria Ananassa ; Root canal dentin.
Subjects: DENTAL > Conservative Dentistry and Endodontics
Depositing User: Subramani R
Date Deposited: 12 Nov 2017 16:56
Last Modified: 12 Nov 2017 16:56
URI: http://repository-tnmgrmu.ac.in/id/eprint/3920

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