Study of Expression of p16 in Oral Squamous Cell Carcinoma, Potentially Malignant Disorders and normal Mucosa.

Sudharsan, R (2013) Study of Expression of p16 in Oral Squamous Cell Carcinoma, Potentially Malignant Disorders and normal Mucosa. Masters thesis, Ragas Dental College and Hospital, Chennai.


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BACKGROUND: It has been hypothesized that inactivation of p16 plays a vital role in evolution of potentially malignant disorders [Epithelial Dysplasia, Oral SubmucousFibrosis (OSF)] and in Oral Squamous Cell Carcinoma (OSCC). We wanted to ascertain the role of p16 in OSF, epithelial dysplasia and OSCC as an early marker for malignant transformation and its liability in OSCC. AIM: To study the expression of p16 in normal mucosa (Group I), OSF (Group II), epithelial dysplasia (Group III) and OSCC (Group IV). MATERIALS AND METHODS: A total sample size of 70 which includes 10 controls of Group I, 20 cases of Group II, 20 cases of Group III, 20 cases of Group IV were studied for p16 expression by immunohistochemistry. We have employed p16 monoclonal antibody of clone G175-405 in our study. The positive control sample of our study is squamous cell carcinoma of uterine cervix. RESULTS: Out of 70 samples 16 cases (22.9%) of cases were p16 positive which includes five in group I, seven in group II, one in group III, three in group IV. Out of sixteen p16 positive samples four cases showed cytoplasmic staining (12,1 in Group I,II,IV respectively) , eleven cases showed nuclear and cytoplasmic staining (3,5,1,2 in Group I,II,III,IV respectively) and one showed cytoplasmic and membrane staining. CONCLUSION: In this study we addressed the association particularly between p16 and OSF, epithelial dysplasia and OSCC. The results of this study with respect to OSF data, highlights that p16 could play a role in malignant transformation of OSF and we hypothesize that it could be associated with HPV.Further studies should ascertain the HPV status of the cases to be included, with a larger sample size to establish and understand if p16 expression could have a role in oral potentially malignant lesions and OSCC.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Tumour suppressor gene p16 ; Oral Submucous Fibrosis ; Oral squamous cell carcinoma ; Epithelial Dysplasia.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 12 Nov 2017 13:45
Last Modified: 12 Nov 2017 13:45

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