Study on Biofilm Forming Ability of Certain Species of Staphylococci and Effect of Antibiotics on Them.

Senthil Raj, R (2010) Study on Biofilm Forming Ability of Certain Species of Staphylococci and Effect of Antibiotics on Them. Masters thesis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore.

[img]
Preview
Text
2606030Senthil Raj R.pdf

Download (1MB) | Preview

Abstract

In our study, biofilm forming ability of Staphylococcal sp. was screened by using Tissue Culture Plate method (TCP), Tube Method (TM) and Congo Red Agar (CRA) methods. The biofilm forming abilities of three Staphylococcal sp. designated S-II, S-III and S-IV were compared with reference strain S-I. S-II exhibited strong biofilm producing ability when studied using all the three methods. The strain S-III exhibited strong biofilm producing ability in TCP and TM method but biofilm producing ability was poor as studied by CRA method. The strain S-IV exhibited moderate biofim producing ability in TCP method but biofilm producing ability was poor in TM and CRA methods. The adhesion process and biofilm formation on biomaterial were determined using 2, 3, 5 Triphenyl Tetrazolium Chloride (TTC) method. All strains were able to form biofilm on biomaterial between 72 to 96 hrs, irrespective of adhesion duration (S-I 12hrs, S-II 24hrs, S-III 24hrs and S-IV 48hrs). The concentration range of Ofloxacin in preventing adhesion process was found to be 3.9 to 62.5μg/ml. Biofilm formation was prevented at 15.6 to 125 μg/ml and to eradicate mature biofilm on biomaterial, 125 to 500 μg/ml was needed against all strains. The concentration range of Rifampin in preventing adhesion process was found to be 0.04 to 0.78μg/ml. Biofilm formation was prevented at 0.78 to 3.12 μg/ml and to eradicate mature biofilm on biomaterial 6.25 to 25 μg/ml concentrations was needed against all strains.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Biofilm Forming Ability; Certain Species; Staphylococci; Antibiotics;
Subjects: PHARMACY > Pharmaceutical Biotechnology
Depositing User: Ravindran C
Date Deposited: 23 Oct 2017 05:32
Last Modified: 23 Oct 2017 05:32
URI: http://repository-tnmgrmu.ac.in/id/eprint/3771

Actions (login required)

View Item View Item