Sivasubramani, R V (2014) Evaluation of the Anticancer Activity of Various Extracts of Evolvulus Alsinoides Linn Using Lung Cancer Cell Line(A549) by Invitro Methods. Masters thesis, Madras Medical College, Chennai.
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Abstract
AIM AND OBJECTIVE: The present study to identify the Phytochemical constituents present in Ethyl acetate, Ethanol and Petroleum ether extract of Evolvulus alsinoides. To evaluate the cytotoxic activity of Ethyl acetate, Ethanol and Petroleum ether extract of Evolvulus alsinoides in human lung cancerous cell line A549 and to establish IC5O concentration. To evaluate the Apoptotic effect of effective extract of Evolvulus alsinoides . To evaluate the Protein levels in the effective extract of Evolvulus alsinoides treated cancerous cell line. To evaluate the Gene expression levels in the effective extract of Evolvulus alsinoides treated cancerous cell line. Cancer is considered as a serious health problem worldwide. Lung cancer remains a major global health problem and leading cause of cancer mortalities in most of the countries in the world with approximately 1.3 million new cases and 300000 deaths each year estimated by World Health Organisation. Lung cancer has been classified into two groups namely small cell and non small cell lung cancer, with the latter being more prevalent accounting for nearly 80% of lung cancer cases.Cancer is considered as a serious health problem worldwide. Lung cancer remains a major global health problem and leading cause of cancer mortalities in most of the countries in the world with approximately 1.3 million new cases and 300000 deaths each year estimated by World Health Organisation. Lung cancer has been classified into two groups namely small cell and non small cell lung cancer, with the latter being more prevalent accounting for nearly 80% of lung cancer cases. With increase in mortality rates among patients suffering from cancer and with limited success being achieved in clinical therapies including radiation, chemotherapy, immune modulation and surgery in treating cancer patients, there arises a need for new way of cancer management. Natural phytochemicals derived from medicinal plants have attained a greater significance in potential management of several diseases including cancer. Several researches have been carried out in evaluation of plant extracts as prophylactic agents which offer greater potential to inhibit carcinogenic process. The mechanism of inhibition of tumour progression by natural phytochemicals range from inhibition of genotoxic effects, increased anti inflammatory and anti oxidant effect, inhibition of cell proliferation, protection of intracellular communications to modulate apoptosis and signal transduction pathways. Discovery of effective herbs and elucidation of their underlying mechanisms could lead to development of an alternative and complimentary method for cancer prevention and treatment. Medicinal plants constitute a common alternative for cancer prevention and treatment in many countries around world. Currently more than 3000 plants around world have been reported to possess anti cancer properties. Screening of different plant components in search of anti cancer drugs is one of the main research activities throughout the world. Vinca alkaloids and cytotoxic podophyllotoxins were discovered in the 1950’s as first anticancer agents from plants. In addition to several plants extracted compounds such as camptothecin, vincristine, vinblastine, taxol and podophyllotoxin , many of the natural compounds were structurally modified to result in stronger anti cancer analogues with less adverse effects. CONCLUSION: Evaluation of Phytochemical analysis of ethyl acetate, ethanol and petroleum ether extracts of Evolvulus alsinoides revealed the presence of proteins, alkaloids, tannins, steroids, phenols and flavanoids. In this present study, Ethyl acetate extract of Evolvulus alsinoides was found to possess potent cytotoxic activity in human lung cancerous cell line A549 and was compared with standard drug 5 Fluorouracil. The Apoptotic effect was confirmed in treated cells by appearance of loss of membrane integrity, leakage of cytoplasmic contents and fragmentation of DNA in treated cells. Its anti cancer effect was further confirmed by reduced levels of proteins Phospho histidine phosphatase and C Reactive Protein in ethyl acetate extract of Evolvulus alsinoides treated cells, whose elevated levels are said to possess a risk in cancer development. Its apoptotic and anti cancer effect may be due to up regulation of genes like p53 and TNF α and down regulation of genes like Bcl-2 and IL-6, which was confirmed by RT-PCR. These results show that ethyl acetate extract of Evolvulus alsinoides possess anti cancer effect and for future perspective, it can be further confirmed by isolating the compounds responsible for the activity and studying the exact mechanism by which the plant possess this activity and confirm the results using in vivo animal models.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Anticancer Activity; Evolvulus Alsinoides Linn; Lung Cancer Cell Line; Invitro Methods |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 23 Oct 2017 05:27 |
| Last Modified: | 27 Oct 2017 08:46 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3768 |
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