Geetha Priya, G (2014) Evaluation of Invitro And Invivo Anti Diabetic Activity of Ethanolic Extract of Portulaca Quadrifida L. on Streptozotocin Induced Diabetes in Rats. Masters thesis, Madras Medical College, Chennai.
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Abstract
AIM AND OBJECTIVES: The present study Phytochemical evaluation of the whole plant of ethanolic extract of Portulaca quadrifida L. To determine the glucose diffusion property of the prepared extract of the Portulaca quadrifida L. using dialysis tube and GOD-POD kit. To evaluate the safety of the effective extract of Portulaca quadrifida L. by acute toxicity study in Wistar albino rats. To investigate the in-vivo anti diabetic effect of active extract of Portulaca quadrifida L. in Streptozotocin induced diabetic Wistar albino rats. In our studies, the damage of pancreas in STZ treated diabetic control (Figure17) and regeneration of β-cells by Glibenclamide (Figure16) was observed. The regeneration in ethanolic extract of Portulaca quadrifida L. was comparable with Glibenclamide; Portulaca quadrifida L. extract (400mg/kg) was showed better regeneration than (200mg/kg) of Portulaca quadrifida L. extract. Photomicrographical data in our studies reinforce healing of pancreas by ethanolic extract of 200mg/kg and 400mg/kg as a plausible mechanism of their antidiabetic activity. The data of our studies suggests that Portulaca quadrifida L. has beneficial effects in diabetes mellitus holding the hope of a new generation for anti-diabetic drugs. CONCLUSION: From the present study we conclude that the preliminary phytochemical analysis of Portulaca quadrifida L. indicated the presence of alkaloids, flavonoids, proteins, saponins, carbohydrates, terpenoids, mucilage, glycosides, tannins and phytosterols. Invitro Glucose adsorption assay of Portulaca quadrifida L. extract was evaluated using GOD-POD kit. Invitro Glucose diffusion properties of Portulaca quadrifida L. extract was evaluated using dialysis membrane and GOD-POD kit. Ethanolic extract of Portulaca quadrifida L. was safe in dose upto 2000mg/kg by acute toxicity study and there was no toxic effects produced. The ethanolic extract of Portulaca quadrifida L. exhibited significant antidiabetic activity in Streptozotocin induced diabetic rats. In vivo studies of Portulaca quadrifida L. ethanolic extract revealed the glucose lowering effect& improved activity of carbohydrate metabolizing enzymes. Thus it was concluded that the ethanolic extract of Portulaca quadrifida L. has anti-diabetic activity.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Invitro; Invivo Anti Diabetic Activity; Ethanolic Extract; Portulaca Quadrifida L; Streptozotocin Induced Diabetes; Rats |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 23 Oct 2017 05:23 |
| Last Modified: | 27 Oct 2017 08:46 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3765 |
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