Abha Yadav, L (2014) Evaluation of Anticancer Activity of Various Extracts of Fruit of Amomum Subulatum Roxb. Using Cervical Cancer Cell Line by Invitro Methods. Masters thesis, College of Pharmacy, Madras Medical College, Chennai.
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Abstract
The present study To identify the Phytochemical constituents of various extracts of fruits of Amomum subulatum To evaluate the cytotoxic activity of ethanolic extract of fruits of Amomum subulatum in cervical cancer cell line HeLa and to establish IC50 concentration. To evaluate the Apoptotic effect of ethanolic extract of fruits of Amomum subulatum. To study the Gene expression levels in Amomum subulatum treated HeLa cancerous cell line. CONCLUSION: Phytochemical analysis of acetone, ethanol and aqueous extracts of Amomum subulatum revealed the presence of proteins, terpenoids, tannins, steroids, phenols and flavonoids. In this study, ethanolic extract of Amomum subulatum showed cytotoxic activity in cervical cancer cell line HeLa by MTT assay. The Apoptotic effect was confirmed by loss of membrane integrity, chromatin condensation, leakage of cytoplasmic contents and fragmentation of DNA by microscopic methods. Its apoptotic and anti cancer effect may be due to up regulation of genes like p53 and down regulation of gene Bcl-2, which was confirmed by RT-PCR. The ethanolic extract of Amomum subulatum possess anti cancer effect and for future perspective, it can be further confirmed by isolating the compounds responsible for the activity and studying the exact mechanism by which the plant possess this activity and confirm the results using in vivo animal models.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Anticancer Activity; Fruit; Amomum Subulatum Roxb; Using Cervical Cancer Cell; Invitro Methods |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 20 Oct 2017 07:23 |
| Last Modified: | 20 Oct 2017 07:23 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3745 |
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