Karthik, R (2012) Pharmacognostical, Phytochemical Pharmacological Evaluation of Justicia Glauca. Masters thesis, College of Pharmacy, Madurai Medical College, Madurai.
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Abstract
The present study the ethno medical information reveals that the species of Justicia possessed a wide spectrum of activity which included anticancer, antirheumatic, antisyphilitic, diuretic, antitubercular, antimalarial, laxative, and sedative, cardio protective, antifungal and antibacterial activities. According to Siddha system of medicine, leaves of Justicia adathoda possess cardio protective property. The ethno medical information also revealed that the fruits of Justicia glauca were found to possess antibacterial and antifungal activities. The phytochemical studies on the leaves have reported for the presence of flavonoids, tannins, alkaloids, sterols and carbohydrates. The cardioprotective, anticancer, antibacterial, antifungal and antioxidant have not yet been reported so far on the whole plant of Justicia glauca Hence the present work has been designed to carry out the following studies on the leaves Justicia glauca Rottler. Pharmacognostical studies on the whole plant. Preliminary phytochemical screening on the extracts of Justicia glauca Quantitative estimation of secondary metabolites. Phytochemical evaluation of whole plant ethanolic extract by TLC and HPTLC studies. CONCLUSION: There are many unknown plants with high medicinal value still not been recognised for their importance. They have not been brought to the light of scientific world. This dissertation covers pharmacognostical, phytochemical and pharmacological studies on the commonly available medicinal plant Justicia glauca belonging to the family Acanthaceae. Pharmacognostical parameters have been determined on the leaves in order to substantiate and identify the plant for future work. Preliminary phytochemical screening carried out on the plant powder and extracts confirmed the presence of flavonoids, phenols, tannins, sterols, alkaloids and carbohydrates The determination of total phenolics (58.06 mg/g), total flavanoid content (29.35mg/g) and total vitamin C content (15.97mg/g) confirms the significant concentration of these phytoconstituents in the ethanolic extract of Justicia glauca . The TLC and HPTLC studies on ethanolic extract of Justicia glauca will help us in the fingerprinting the profile of the plant for future reference. The pharmacological screening confirms The ethanolic extract of Justicia glauca have exhibited radical scavenging activity by DPPH assay and hydrogen peroxide method shown potent antioxidant activity. Significant cardioprotective activity at 100mg/kg of S (p<0.001), 200 mg/kg of EJS (p<0.001) against Doxorubicin induced cardiotoxicity in rats and the in vivo antioxidant activity of these extracts may be ascribed for this cardioprotective activity which is confirmed by elevation of GSH (p<0.001)and reduction of TBARS (p<0.001) in the tissue parameters of the treated animals. Significant anti-bacterial activity exhibited by ethanolic extract (1.5mg/ disc) against Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Staphylococcus aureus, Staphylococcus albus and Pseudomonas aeruginosa. Significant anti-fungal activity exhibited by ethanolic extract (8/ disc) against Aspergillus flavus and Candida albicans. It was evident from the phytochemical studies of this plant, that substantial amount of phenols and flavanoids were present in these extracts which exhibited significant invitro antioxidant activity. Cardiomyopathy may be due to viruses, amyloidosis, diabetes, thyroid diseases, medications such as chemotheraphy drugs (Doxorubicin, Donorubicin, etc). Though many synthetic cardioprotective agents are available, herbal drugs are preferred for their safety and efficacy. Cardiotoxicity was induced in the animal model using Doxorubicin (antineoplastic agent). Doxorubicin cause oxidative stress leads to cardiac tissue damage at 15mg/kg i.p in rats. The extract has shown significant cardioprotective action and anti-bacterial activity against the pathogenic organisms. The ethanolic extract of Justicia glauca may serve as a lead medicinal plant to synthesise various semi-synthetic drugs to treat various life threatening diseases like congestive heart failure, cancer, bacterial. fungal infections.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Pharmacognostical; Phytochemical Pharmacological Evaluation; Justicia Glauca |
| Subjects: | PHARMACY > Pharmacognosy |
| Depositing User: | Ravindran C |
| Date Deposited: | 20 Oct 2017 07:12 |
| Last Modified: | 20 Oct 2017 07:12 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3725 |
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