Senthilkumar, I (2014) Quantification of Chlorthalidone in Bulk and Pharmaceutical Dosage form by UV, Infrared Spectrophotometry and Spectrofluorimetry. Masters thesis, College of Pharmacy, Madras Medical College, Chennai.
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Abstract
The present study Chlorthalidone is an official drug of IP/BP and USP , Review of literature indicates that several methods have been reported for the estimation of chlorthalidone by UV-Visible spectrophotometric and RP-HPLC. The aim of the present work is to develop and validated simple, novel,sensitive,highly specific,accurate and precise UV-Visible, Infrared spectrophotometry and spectrofluorimetry methods for the estimation of chlorthalidone in bulk and tablet dosage form which has not been reported till date. The novel analytical methods comprises of : UV Spectrophotometry Standard absorbance method Area under the curve First derivative spectroscopy Second derivative spectroscopy Q-Absorbance method Infrared Spectrophotometry KBr Disc method using Internal standard. Spectrofluorimetry Direct spectrofluorimetric method. CONCLUSION: The Ultraviolet method involves the determination of chlorthalidone by different methods like standard absorbance method ,area under curve, derivative spectroscopy and Q-absorbance method. The drug obeyed Beer’s law at the concentration of 40 -160 μg/ml. The correlation coefficient was found to be 0.998 for all the methods. The low percentage RSD value shows that the methods developed are not affected by the presence of sample matrix (or) devoid of interference by the excipients. The quantitative infrared spectrophotometric method is a novel method where KSCN has been used as the internal standard. The drug obeyed Beer’s law in the concentration range of 0.5mg- 2.0mg. The drug showed good linearity as indicated by correlation coefficient value 0.9942. The low percentage RSD value shows that the methods developed are not affected by the presence of sample matrix (or) devoid of interference by the excipients. The quantitative spectrofluorimetric methods for estimation of Chlorthalidone is also a novel method. Chlorthalidone was found to exhibit fluorescence in the concentration range of 0.4 - 2.0μg/ml by direct fluorimetric method. Chlorthalidone showed good linearity as indicated by correlation coefficient value of 0.9938 respectively. Thus the methods were precise, sensitive, highly specific and accurate.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Pharmaceutical Dosage; UV Infrared Spectrophotometry; Spectrofluorimetry |
| Subjects: | PHARMACY > Pharmaceutical Chemistry |
| Depositing User: | Ravindran C |
| Date Deposited: | 20 Oct 2017 07:01 |
| Last Modified: | 20 Oct 2017 07:01 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3716 |
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