Kalaimagal, A (2012) Quantification of Valsartan by Uv-Visible and NMR Spectroscopy. Masters thesis, College of Pharmacy, Madras Medical College, Chennai.
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Abstract
The Present study standard analytical techniques are not available in the pharmacopoeias for newer drugs. So it becomes necessary to develop new analytical techniques for the newer drugs. Valsartan is one of the new drugs launched recently. The review of literature reveals that analytical techniques like HPLC, HPTLC, LC/MS have been reported for valsartan individually and in combination with other drugs but there is no evidence for the study of valsartan by UV using 0.1N sodium hydroxide, colorimetry involving acid dye method and quantitative NMR. Thus the main objective of the work is to develop a simple, easy to perform, cost effective, accurate, precise and validated UV-Visible and qNMR methods for the estimation of Valsartan in bulk and tablet dosage forms. The methods developed include: UV Spectroscopy, Standard comparison method, area Under the Curve, Derivative spectroscopy (First derivative, Second derivative), Q-Absorbance Method, Visible Spectroscopy- Acid dye method, Bromophenol and phosphate buffer pH 4 Bromothymol blue and phosphate buffer pH 4. CONCLUSION:The present work entitled “Quantification of Valsartan by UV-Visible and NMR spectroscopy comprises of UV-Visible spectrophotometric method and QNMR methods for estimation of Valsartan. The Ultraviolet method involves the determination of Valsartan by different methods like standard absorbance method, area under curve, derivative spectroscopy and Q-absorbance method. The drug obeyed Beer’s law at the concentration of 5-30μg/mL. The correlation coefficient was found to be 0.99 for all the method. The low percentage RSD value shows that the methods developed are not affected by the presence of sample matrix (or) devoid of interference by the excipients. The visible method involve Acid dye complexation with Bromo thymol blue and Bromo phenol blue. Both the methods obeyed Beer’s law at the concentration of 100-600μg/mL for Bromo thymol blue and 40-240μg/mL for Bromo phenol blue. The correlation coefficients were within the limit, and RSD percentage was low. Thus the methods were precise, specific and accurate. The qNMR method of Quantification is a novel and sophisticated technique. TCNB has been used as the internal standard and the solvent used is CDCL3. The intergrated intensity of resonance of valsartan at 1.73 ppm and 4.9ppm were considered for the Quantification. The standard and the sample resonance were compared and the amount present was calculated. The precision studies and the recovery studies were performed. This method was precise and accurate though makes use of sophisticated and costlier instrument.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Valsartan; Uv-Visible; NMR Spectroscopy |
| Subjects: | PHARMACY > Pharmaceutical Chemistry |
| Depositing User: | Ravindran C |
| Date Deposited: | 20 Oct 2017 06:43 |
| Last Modified: | 20 Oct 2017 06:43 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3705 |
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